Product Info Summary
SKU: | EK1706 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FGFBP1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1706
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FGFBP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human FGFBP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FGFBP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1706)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: K24-C234
Sensitivity
<5 pg/ml
Assay Range
23.4 pg/ml - 1500 pg/ml
Standard Dilution Instructions
See datasheet of EK1706 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1706 is reactive to FGFBP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1706 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF-BP
Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1706-CAP | Anti-Human FGFBP1 Pre-coated 96-well strip microplate | 1 |
EK1706-ST | Human FGFBP1 Standard | 2 vials, 10 ng/tube |
EK1706-DA | Human FGFBP1 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 23.4 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 |
O.D. | 0.088 | 0.103 | 0.216 | 0.439 | 0.704 | 1.146 | 1.588 | 2.114 |
Data Example Images
Click image to see more details
Human FGFBP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF-BP, Dilution ratio of 1:10, concentration in serum and plasma is around 1.7 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FGFBP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 29 | 158 | 934 | 31 | 160 | 978 |
Standard deviation | 1.83 | 9.01 | 44.83 | 2.05 | 11.68 | 75.3 |
CV (%) | 6.3% | 5.7% | 4.8% | 6.6% | 7.3% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FGF-BP in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 29 | 29 | 32 | 33 | 30 | 1.78 | 5.9% |
Sample . 2 | 158 | 181 | 166 | 164 | 167 | 8.46 | 5.0%% |
Sample 3 | 934 | 866 | 979 | 830 | 902 | 57.94 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For FGFBP1 (Source: Uniprot.Org, NCBI)
Gene Name
FGFBP1
Full Name
Fibroblast growth factor-binding protein 1
Weight
26.264kDa
Superfamily
fibroblast growth factor-binding protein family
Alternative Names
Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGF-binding protein 1; FGF-binding protein 1; 17 kDa HBGF-binding protein; 17 kDa HBGF-binding protein; 17 kDa HBGF-binding protein; FGFBP; HBP17 FGFBP1 FGF-BP, FGF-BP1, FGFBP, FGFBP-1, HBP17 fibroblast growth factor binding protein 1 fibroblast growth factor-binding protein 1|17 kDa HBGF-binding protein|17 kDa heparin-binding growth factor-binding protein|FGF-binding protein 1|heparin-binding growth factor binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FGFBP1, check out the FGFBP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGFBP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FGFBP1 ELISA Kit PicoKine® (EK1706)
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No publications found for EK1706
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human FGFBP1 ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of FGF-BP in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
D. Banerjee
Verified customer
Asked: 2020-01-31
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FGF-BP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-31
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Walker
Verified customer
Asked: 2018-07-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-07-04
Question
Q: how to analyze ELISA data? I have performed FGF-BP level in serum.
B. Mehta
Verified customer
Asked: 2017-05-13
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-05-13
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
B. Perez
Verified customer
Asked: 2017-04-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-04-18
Question
Q: Can FGF-BP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-10-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-10-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
G. Edwards
Verified customer
Asked: 2016-02-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-02-25