Anti-Estrogen receptor ESR1 Antibody

Product Name

Anti-Estrogen receptor ESR1 Antibody

View all ER alpha/NR3A1 Antibodies

SKU/Catalog Number

A00057

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Estrogen receptor ESR1 Antibody (Catalog # A00057). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Estrogen receptor ESR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00057)

Host

Rabbit

Contents

ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1. The immunogen is located within amino acids 250 - 300 of ESR1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ESR1 antibody is human, mouse and rat reactive.

Reactive Species

A00057 is reactive to ESR1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

66216 MW

Background of ER alpha/NR3A1

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00057 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)

Alternative Names

ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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