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Product Info Summary
| SKU: | P00057 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-ER alpha (S118) ESR1 Rabbit Monoclonal Antibody
View all ER alpha/NR3A1 Antibodies
SKU/Catalog Number
P00057
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-ER alpha (S118) ESR1 Rabbit Monoclonal Antibody catalog # P00057. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ER alpha (S118) ESR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00057)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EIG-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ER alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00057 is reactive to ESR1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
66 kDa
Calculated molecular weight
66216 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00057 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human kidney tissue
ICC/IF: MCF7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ER/ESR1 (Phospho-S118) using anti-ER/ESR1 (Phospho-S118) antibody (P00057).
Electrophoresis was performed on a 0.1 SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ER/ESR1 (Phospho-S118) antigen affinity purified monoclonal antibody (P00057) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ER/ESR1 (Phospho-S118) at approximately 66 kDa. The expected band size for ER/ESR1 (Phospho-S118) is at 66 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-ER alpha (S118) Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of MCF7 cells treated with EGF, using Phospho-ER alpha (S118) Antibody.
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Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF.
Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1; ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ER alpha (S118) ESR1 Rabbit Monoclonal Antibody (P00057)
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