Anti-Clusterin Purified Monoclonal Antibody

Product Name

Anti-Clusterin Purified Monoclonal Antibody

View all Clusterin/APOJ Antibodies

SKU/Catalog Number

M00590-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Clusterin Purified Monoclonal Antibody (Catalog# M00590-1). Tested in WB, IHC-P, ICC, ELISA application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Clusterin Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00590-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

Hs-3

Isotype

Mouse IgG1

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J), a conserved secreted glycoprotein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00590-1 is reactive to CLU in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

52495 MW

Background of Clusterin/APOJ

Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00590-1 is guaranteed for ELISA, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: Sertoli cells.
Western blotting: 1 μg/ml; positive material: seminal plasma.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CLU (Source: Uniprot.org, NCBI)

Alternative Names

Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, CLU, NA1/NA2, Apolipoprotein J, Apo-J, TRPM-2, KBP1, KUB1, CLI, AAG4, APOJ, SGP2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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