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Product Info Summary
| SKU: | EK1615 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat Clusterin ELISA Kit PicoKine®
View all Clusterin/APOJ ELISA kits
SKU/Catalog Number
EK1615
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Clusterin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Clu in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Clusterin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1615)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
Sensitivity
<15 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1615 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1615 is reactive to Clu in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1615 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Clusterin/APOJ
Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1615-CAP | Anti-Rat Clu Pre-coated 96-well strip microplate | 1 |
| EK1615-ST | Rat Clu Standard | 2 vials, 20 ng/tube |
| EK1615-DA | Rat Clu Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.007 | 0.082 | 0.164 | 0.264 | 0.521 | 0.984 | 1.692 | 2.055 |
Data Example Images
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Rat Clusterin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Clusterin/APOJ, Dilution ratio of 1:20000, concentration in serum and plasma is around 80 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Clusterin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 428 | 2367 | 10471 | 423 | 2436 | 10928 |
| Standard deviation | 19.26 | 120.71 | 827.2 | 21.57 | 160.77 | 1060.01 |
| CV (%) | 4.5% | 9.7% | 7.9% | 9.7% | 6.6% | 9.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Clusterin/APOJ in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 428 | 422 | 418 | 423 | 422 | 3.56 | 0.8% |
| Sample 2 | 2367 | 2282 | 2285 | 2383 | 2329 | 46.11 | 1.9% |
| Sample 3 | 10471 | 10267 | 10049 | 10442 | 10307 | 168.28 | 1.6% |
Protein Target Info & Infographic
Gene/Protein Information For Clu (Source: Uniprot.Org, NCBI)
Gene Name
Clu
Full Name
Clusterin
Weight
52.495kDa
Superfamily
clusterin family
Alternative Names
Clusterin; Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2; Clusterin beta chain; Clusterin alpha chain; Clu; CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Clu, check out the Clu Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Clu: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Clusterin ELISA Kit PicoKine® (EK1615)
Hello CJ!
EK1615 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of the differentially expressed protein biomarkers in rat blood plasma in response to gamma irradiation
Identification of the differentially expressed protein biomarkers in rat blood plasma in response to gamma irradiation
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Customer Q&As
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6 Customer Q&As for Rat Clusterin ELISA Kit PicoKine®
Question
Does EK1615 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Rat Clusterin ELISA Kit PicoKine® (EK1615) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-09-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-20
Question
Q: how can I thaw whole blood sample for Clusterin ELISA after freezing?
D. Young
Verified customer
Asked: 2020-07-16
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Clusterin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-07-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Y. Banerjee
Verified customer
Asked: 2020-04-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-23
Question
Q: What is the optimal O.D. value for Clusterin ELISA kit? I used your Clusterin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Clusterin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Clusterin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Clusterin that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2018-11-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-19
