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Product Info Summary
| SKU: | EKC1202 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit
See all ER alpha/NR3A1 products
SKU/Catalog Number
EKC1202
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Estrogen Receptor-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Estrogen Receptor-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of Estrogen Receptor-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Estrogen Receptor-alpha.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1202)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1202 is reactive to ESR1 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1202 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Estrogen Receptor-alpha Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells
Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.Org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
ER;ESR;ESR1;ESTR;ESTRA;Estradiol receptor;Estrogen receptor;NR3A1 ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ESR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit (EKC1202)
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Customer Q&As
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2 Customer Q&As for Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit
Question
Dear Technical Team: I planned to perform fluorescent immune-staining with two estrogen receptor antibodies on the same sample tissue slide. In your catalogue online I found Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit as the most compatible primary antibodies. However, I need more information about immunostaining, can you provide me with more details about the dilutions and the method that I will used to performed simultaneous fluorescent immunostaining.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Our product Estrogen Receptor-alpha Colorimetric Cell-Based ELISA Kit has been tested for IHC-P, so definitely it will work in immunofluorescence. Since the host species and the isotypes are different, they can be applied in double staining with compatible secondary antibodies. Additionally, we would suggest before you perform double staining, try to use first the antibodies in single staining to make sure that the procedure works well. When the results detects two targets nicely, then you can proceed with the double staining on the same samples.
Boster Scientific Support
Answered: 2020-01-17
Question
Is the antibody EKC1202 amino acids correspond to beta estradiol?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
We are not sure if the antibody EKC1202 amino acids would correspond with beta estradiol, though the immunogen that was used to generate the antibody is recombinant fragment that correspond to amino acids 302-595 of Human Estrogen Receptor. Thus, you could possibly align this section of the human estrogen receptor with your target sequence and check the level of similarity.
Boster Scientific Support
Answered: 2017-09-25
