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Product Info Summary
| SKU: | M00057-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
View all ER alpha/NR3A1 Antibodies
SKU/Catalog Number
M00057-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ER alpha ESR1 Rabbit Monoclonal Antibody catalog # M00057-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ER alpha ESR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IE-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ER alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00057-2 is reactive to ESR1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
82 kDa
Calculated molecular weight
66216 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00057-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
FC 1:30
Positive Control
IHC: human cervix carcinoma tissue
ICC/IF: MCF7 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.
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Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody.
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Immunofluorescent analysis of MCF7 cells, using ER alpha Antibody .
Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1; ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ER alpha ESR1 Rabbit Monoclonal Antibody (M00057-2)
Hello CJ!
M00057-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of estradiol on liver estrogen receptor-? and its mRNA expression in hepatic fibrosis in rats
Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
Question
What is a secondary antibody for the M00057-2 antibody that is HRP or biotin conjugate?
Verified customer
Asked: 2019-11-04
Answer
For the Anti-ER alpha Rabbit Monoclonal Antibody M00057-2, we suggest Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate BA1054 and Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugate BA1003 as secondary antibodies that is HRP (Horseradish Peroxidase) or biotin conjugate.
Boster Scientific Support
Answered: 2019-11-04
Question
For M00057-2 (Abways CY5041) , what is the immunogen sequence?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The immunogen sequence for M00057-2 (Abways CY5041) is CPPLNSVSPSPLMLLHPPPQ.
Boster Scientific Support
Answered: 2017-11-13
