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Product Info Summary
| SKU: | PB10052 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-CD40 Antibody Picoband®
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
PB10052
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD40 Antibody Picoband® catalog # PB10052. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10052)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10052 is reactive to Cd40 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
32093 MW
Background of CD40/TNFRSF5
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10052 is guaranteed for ELISA, Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Positive Control
WB: mouse spleen tissue
IHC: mouse lymphaden tissue
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 2. IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052).
CD40/TNFRSF5 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10052) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 40 kDa. The expected band size for CD40/TNFRSF5 is at 32 kDa.
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3. Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (PB10052).
Overlay histogram showing U937 cells stained with PB10052 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (PB10052,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Cd40 (Source: Uniprot.org, NCBI)
Gene Name
Cd40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
32093 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CD40;Cd40;Tnfrsf5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd40, check out the Cd40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40 Antibody Picoband® (PB10052)
Hello CJ!
PB10052 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium
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Customer Q&As
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16 Customer Q&As for Anti-CD40 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-CD40 antibody PB10052. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-05
Question
We are currently using anti-CD40 antibody PB10052 for mouse tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-CD40 antibody (PB10052) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
I am looking for using your anti-CD40 antibody for defense response to protozoan studies. Has this antibody been tested with western blotting on u937 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate your inquiry. This PB10052 anti-CD40 antibody is tested on u937 cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-04
Question
Do you have a BSA free version of anti-CD40 antibody PB10052 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD40 antibody PB10052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
Our lab used your anti-CD40 antibody for IHC on left coronary artery in a previous experiment. I am using human, and We intend to use the antibody for Flow Cytometry next. We are interested in examining left coronary artery as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
I viewed the website and datasheets of our anti-CD40 antibody and I see that PB10052 has been validated on human in both IHC and Flow Cytometry. Thus PB10052 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-05
Question
Can you help my question with product PB10052, anti-CD40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10052 anti-CD40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
Our lab were satisfied with the WB result of your anti-CD40 antibody. However we have seen positive staining in leukocyte isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
From literature, leukocyte does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2019-11-18
Question
Would PB10052 anti-CD40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
It shows on the product datasheet, PB10052 anti-CD40 antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-20
Question
We have observed staining in human ovary. What should we do? Is anti-CD40 antibody supposed to stain ovary positively?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
From what I have seen in literature ovary does express CD40. From what I have seen in Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2019-07-19
Question
I was wanting to use your anti-CD40 antibody for Flow Cytometry for mouse left coronary artery on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse left coronary artery identification?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
As indicated on the product datasheet, PB10052 anti-CD40 antibody has been tested for ELISA, Flow Cytometry, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse left coronary artery in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-06
Question
Is this PB10052 anti-CD40 antibody reactive to the isotypes of CD40?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
The immunogen of PB10052 anti-CD40 antibody is E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-15
Question
I see that the anti-CD40 antibody PB10052 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
V. Williams
Verified customer
Asked: 2018-03-08
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-08
Question
Is a blocking peptide available for product anti-CD40 antibody (PB10052)?
K. Patel
Verified customer
Asked: 2018-01-04
Answer
We do provide the blocking peptide for product anti-CD40 antibody (PB10052). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-04
Question
Here is the WB image, lot number and protocol we used for left coronary artery using anti-CD40 antibody PB10052. Please let me know if you require anything else.
B. Moore
Verified customer
Asked: 2016-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-01
Question
Will anti-CD40 antibody PB10052 work for Flow Cytometry with left coronary artery?
J. Parker
Verified customer
Asked: 2015-03-10
Answer
According to the expression profile of left coronary artery, CD40 is highly expressed in left coronary artery. So, it is likely that anti-CD40 antibody PB10052 will work for Flow Cytometry with left coronary artery.
Boster Scientific Support
Answered: 2015-03-10
Question
I am interested in to test anti-CD40 antibody PB10052 on mouse left coronary artery for research purposes, then I may be interested in using anti-CD40 antibody PB10052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Anderson
Verified customer
Asked: 2013-04-01
Answer
The products we sell, including anti-CD40 antibody PB10052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-01
