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Product Info Summary
| SKU: | PB9140 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD11b/ITGAM Antibody Picoband®
SKU/Catalog Number
PB9140
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD11b/ITGAM Antibody Picoband® catalog # PB9140. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b/ITGAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9140)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9140 is reactive to ITGAM in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
127179 MW
Background of ITGAM
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9140 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
IHC: human tonsil tissue, mouse spleen tissue, rat spleen tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (PB9140).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CD11B protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # PB9140) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 45 kDa. The expected band size for CD11b is at 45 kDa.
Click image to see more details
Figure 2. IHC analysis of CD11b using anti-CD11b antibody (PB9140).
CD11b was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD11b using anti-CD11b antibody (PB9140).
CD11b was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD11b using anti-CD11b antibody (PB9140).
CD11b was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of THP-1 cells using anti-CD11b antibody (PB9140).
Overlay histogram showing THP-1 cells stained with PB9140 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (PB9140,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127179 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b/ITGAM Antibody Picoband® (PB9140)
Hello CJ!
PB9140 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy
The Neurovascular Protective Effects of Huperzine A on D-Galactose-Induced Inflammatory Damage in the Rat Hippocampus
Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Ren B, Zhang Yx, Zhou Hx, Sun Fw, Zhang Zf, Wei Z, Zhang Cy, Si Dw. J Neurol Sci. 2015 Jan 15;348(1-2):142-52. Doi: 10.1016/J.Jns.2014.11.026. Epub 2014 Nov 28. Tanshinone Iia Prevents The Loss Of Nigrostriatal Dopaminergic Neurons By Inhibiting N...
Zhao G, Wang S, Wang Z, Sun A, Yang X, Qiu Z, Wu C, Zhang W, Li H, Zhang Y, Zhao J, Zou Y, Ge J. Int J Cardiol. 2013 Sep 30;168(2):853-62. Doi: 10.1016/J.Ijcard.2012.10.022. Epub 2012 Nov 13. Cxcr6 Deficiency Ameliorated Myocardial Ischemia/Reperf...
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-CD11b/ITGAM Antibody Picoband®
Question
Is a blocking peptide available for product anti-CD11b/ITGAM antibody (PB9140)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
We do provide the blocking peptide for product anti-CD11b/ITGAM antibody (PB9140). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-02
Question
you antibody to test anti-CD11b/ITGAM antibody PB9140 on mouse blood for research purposes, then I may be interested in using anti-CD11b/ITGAM antibody PB9140 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-CD11b/ITGAM antibody PB9140, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Here is the WB image, lot number and protocol we used for blood using anti-CD11b/ITGAM antibody PB9140. Please let me know if you require anything else.
M. Singh
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD11b/ITGAM antibody PB9140. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
Can you share the antigen retrieval method for IHC (P) sections used in your PB9140 antibody?
Verified customer
Asked: 2020-01-08
Answer
For Anti-CD11b/ITGAM Antibody Picoband PB9140, the following are the steps in antigen retrieval method. Heat-induced epitope retrieval: 1) Immerse the slides in antigen retrieval buffer (EDTA buffer, pH 8.0). 2) Microwave the solution at MED HI power for 8 min. 3) Cool the slides for 5 min. 4) Then microwave the solution at HIGH power for 4 min. 5) Cool the slides to room temperature.
Boster Scientific Support
Answered: 2020-01-10
Question
Will anti-CD11b/ITGAM antibody PB9140 work for ICC with blood?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to the expression profile of blood, ITGAM is highly expressed in blood. So, it is likely that anti-CD11b/ITGAM antibody PB9140 will work for ICC with blood.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this PB9140 anti-CD11b/ITGAM antibody reactive to the isotypes of ITGAM?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The immunogen of PB9140 anti-CD11b/ITGAM antibody is E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-25
Question
I was wanting to use your anti-CD11b/ITGAM antibody for ICC for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
It shows on the product datasheet, PB9140 anti-CD11b/ITGAM antibody has been validated for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-CD11b/ITGAM antibody PB9140 for rat tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-CD11b/ITGAM antibody (PB9140) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
We have been able to see staining in human blood. What should we do? Is anti-CD11b/ITGAM antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
Based on literature blood does express ITGAM. Based on Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-CD11b/ITGAM antibody PB9140 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-21
Question
Would PB9140 anti-CD11b/ITGAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can see on the product datasheet, PB9140 anti-CD11b/ITGAM antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-26
Question
Is PB9140 and A00144-1 suitable for IF application?
Verified customer
Asked: 2019-03-26
Answer
IF test results of the Anti-CD11b/ITGAM Antibody Picoband™ (PB9140) and Anti-CD11b/ITGAM Antibody Picoband™ (A00144-1) were not good. We do not recommend using the antibodies for IF application.
Boster Scientific Support
Answered: 2019-03-26
Question
I have a question about product PB9140, anti-CD11b/ITGAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Parker
Verified customer
Asked: 2017-07-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9140 anti-CD11b/ITGAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-04
Question
Is there a BSA free version of anti-CD11b/ITGAM antibody PB9140 available?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11b/ITGAM antibody PB9140 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-08
Question
We purchased anti-CD11b/ITGAM antibody for ICC on blood in a previous project. I am using mouse, and We want to use the antibody for WB next. I would like examining blood as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
R. Dhar
Verified customer
Asked: 2016-08-29
Answer
I have checked the website and datasheets of our anti-CD11b/ITGAM antibody and I see that PB9140 has been tested on mouse in both ICC and WB. Thus PB9140 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-08-29
Question
We are interested in using your anti-CD11b/ITGAM antibody for receptor-mediated endocytosis studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
A. Li
Verified customer
Asked: 2015-10-13
Answer
Thank you for your inquiry. This PB9140 anti-CD11b/ITGAM antibody is validated on human tonsil tissue, mouse spleen tissue, rat spleen tissue. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-13
Question
My team were well pleased with the WB result of your anti-CD11b/ITGAM antibody. However we have seen positive staining in bone marrow cell cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
E. Taylor
Verified customer
Asked: 2013-07-16
Answer
From literature, bone marrow cell does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2013-07-16
