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Product Info Summary
| SKU: | PA2176 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CCR3 Antibody Picoband®
SKU/Catalog Number
PA2176
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR3 Antibody catalog # PA2176. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2176)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CCR3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2176 is reactive to CCR3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated molecular weight
41044 MW
Background of CCR3
C-C chemokine receptor type 3, also called CCR3 or CKR3 is a protein that in humans is encoded by the CCR3 gene. The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2176 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: K562 Cell, RAJI Cell
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-CCR3 antibody, PA2176, Western blotting
Lane 1: K562 Cell Lysate
Lane 2: RAJI Cell Lysate
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Anti-CCR3 antibody, PA2176, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For CCR3 (Source: Uniprot.org, NCBI)
Gene Name
CCR3
Full Name
C-C chemokine receptor type 3
Weight
41044 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 3;C-C CKR-3;CC-CKR-3;CCR-3;CCR3;CKR3;Eosinophil eotaxin receptor;CD193;CCR3;CMKBR3; CCR3 C C CKR3, CC-CKR-3, CD193, CKR 3, CKR3, CMKBR3 C-C motif chemokine receptor 3 C-C chemokine receptor type 3|C-C CKR-3|CC chemokine receptor 3|CCR-3|b-chemokine receptor|chemokine (C-C motif) receptor 3|eosinophil CC chemokine receptor 3|eosinophil eotaxin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR3, check out the CCR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR3 Antibody Picoband® (PA2176)
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No publications found for PA2176
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CCR3 Antibody Picoband®
Question
My question regards to test anti-CCR3 antibody PA2176 on human blood for research purposes, then I may be interested in using anti-CCR3 antibody PA2176 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The products we sell, including anti-CCR3 antibody PA2176, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-CCR3 antibody PA2176 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-03
Question
Here is the WB image, lot number and protocol we used for blood using anti-CCR3 antibody PA2176. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-CCR3 antibody PA2176 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The anti-CCR3 antibody (PA2176) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
you antibody using your anti-CCR3 antibody for chemokine receptors bind chemokines studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you for your inquiry. This PA2176 anti-CCR3 antibody is tested on k562 cell lysate, raji cell lysate, human tonsil tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-30
Question
We purchased anti-CCR3 antibody for IHC on synovium a few months ago. I am using human, and We intend to use the antibody for WB next. We want examining synovium as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
E. Johnson
Verified customer
Asked: 2019-10-21
Answer
I looked at the website and datasheets of our anti-CCR3 antibody and I see that PA2176 has been tested on human in both IHC and WB. Thus PA2176 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CCR3 antibody PA2176. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-23
Question
I have a question about product PA2176, anti-CCR3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Huang
Verified customer
Asked: 2019-09-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2176 anti-CCR3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this PA2176 anti-CCR3 antibody reactive to the isotypes of CCR3?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The immunogen of PA2176 anti-CCR3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CCR3(1-20aa MTTSLDTVETFGTTSYYDDV). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-19
Question
Is a blocking peptide available for product anti-CCR3 antibody (PA2176)?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
We do provide the blocking peptide for product anti-CCR3 antibody (PA2176). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-21
Question
Does anti-CCR3 antibody PA2176 work on monkey IHC with monocyte?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
Our lab technicians have not validated anti-CCR3 antibody PA2176 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CCR3 antibody PA2176 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey monocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-12
Question
Would PA2176 anti-CCR3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
It shows on the product datasheet, PA2176 anti-CCR3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-10
Question
Is there a BSA free version of anti-CCR3 antibody PA2176 available?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCR3 antibody PA2176 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-23
Question
I was wanting to use your anti-CCR3 antibody for IHC for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
As indicated on the product datasheet, PA2176 anti-CCR3 antibody has been tested for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-25
Question
Does anti-CCR3 antibody PA2176 work for IHC with blood?
C. Williams
Verified customer
Asked: 2016-02-25
Answer
According to the expression profile of blood, CCR3 is highly expressed in blood. So, it is likely that anti-CCR3 antibody PA2176 will work for IHC with blood.
Boster Scientific Support
Answered: 2016-02-25
Question
We have been able to see staining in human monocyte. Any tips? Is anti-CCR3 antibody supposed to stain monocyte positively?
R. Gonzalez
Verified customer
Asked: 2015-06-30
Answer
From literature monocyte does express CCR3. From Uniprot.org, CCR3 is expressed in blood, monocyte, synovium, among other tissues. Regarding which tissues have CCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7622448
Synovium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-06-30
Question
My boss were happy with the WB result of your anti-CCR3 antibody. However we have been able to see positive staining in synovium cell membrane using this antibody. Is that expected? Could you tell me where is CCR3 supposed to be expressed?
B. Li
Verified customer
Asked: 2013-03-05
Answer
From literature, synovium does express CCR3. Generally CCR3 expresses in cell membrane. Regarding which tissues have CCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7622448
Synovium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-03-05
