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Product Info Summary
| SKU: | A01438 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Eotaxin CCL11 Antibody
View all CCL11/Eotaxin Antibodies
SKU/Catalog Number
A01438
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Eotaxin CCL11 Antibody (Catalog # A01438). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
Eotaxin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Eotaxin CCL11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01438)
Host
Rabbit
Contents
Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin. The immunogen is located within the last 50 amino acids of Eotaxin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.
Reactive Species
A01438 is reactive to CCL11 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
10732 MW
Background of CCL11/Eotaxin
Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01438 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 μg/mL.
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Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 μg/mL.
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Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CCL11 (Source: Uniprot.org, NCBI)
Gene Name
CCL11
Full Name
Eotaxin
Weight
10732 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
SCYA11, SCYA11, Eotaxin, C-C motif chemokine 11 CCL11 SCYA11 C-C motif chemokine ligand 11 eotaxin|chemokine (C-C motif) ligand 11|eosinophil chemotactic protein|eotaxin-1|small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL11, check out the CCL11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Eotaxin CCL11 Antibody (A01438)
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