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Product Info Summary
| SKU: | A02549 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Histamine H4 Receptor HRH4 Antibody
View all Histamine H4R Antibodies
SKU/Catalog Number
A02549
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Histamine H4 Receptor HRH4 Antibody catalog # A02549. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histamine H4 Receptor HRH4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02549)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human HRH4. AA range:221-270
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02549 is reactive to HRH4 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
44496 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02549 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of COLO205 cells using Histamine H4 Receptor Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HRH4 (Source: Uniprot.org, NCBI)
Gene Name
HRH4
Full Name
Histamine H4 receptor
Weight
44496 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Histamine H4 receptor;H4R;HH4R;AXOR35;G-protein coupled receptor 105;GPRv53;Pfi-013;SP9144;HRH4;GPCR105; HRH4 AXOR35, BG26, GPCR105, GPRv53, H4, H4R, HH4R histamine receptor H4 histamine H4 receptor|G-protein coupled receptor 105|SP9144|pfi-013
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HRH4, check out the HRH4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HRH4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histamine H4 Receptor HRH4 Antibody (A02549)
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No publications found for A02549
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Histamine H4 Receptor HRH4 Antibody
Question
We are currently using anti-Histamine H4 Receptor antibody A02549 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The anti-Histamine H4 Receptor antibody (A02549) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-08
Question
Does anti-Histamine H4 Receptor antibody A02549 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
According to the expression profile of leukocyte, HRH4 is highly expressed in leukocyte. So, it is likely that anti-Histamine H4 Receptor antibody A02549 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2020-03-27
Question
Would A02549 anti-Histamine H4 Receptor antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Li
Verified customer
Asked: 2020-03-09
Answer
As indicated on the product datasheet, A02549 anti-Histamine H4 Receptor antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-Histamine H4 Receptor antibody A02549 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-07
Question
Is a blocking peptide available for product anti-Histamine H4 Receptor antibody (A02549)?
C. Banerjee
Verified customer
Asked: 2016-10-04
Answer
We do provide the blocking peptide for product anti-Histamine H4 Receptor antibody (A02549). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-10-04
Question
Is this A02549 anti-Histamine H4 Receptor antibody reactive to the isotypes of HRH4?
P. Jones
Verified customer
Asked: 2014-02-11
Answer
The immunogen of A02549 anti-Histamine H4 Receptor antibody is Synthesized peptide derived from the Internal region of human Histamine H4 Receptor. at AA rangle: 190-270. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-11
