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Product Info Summary
| SKU: | PA1013-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Apoptosis regulator BAX Bax Antibody Picoband®
SKU/Catalog Number
PA1013-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apoptosis regulator BAX Bax Antibody catalog # PA1013-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apoptosis regulator BAX Bax Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1013-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Bax, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1013-1 is reactive to BAX in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa, 28 kDa
Calculated molecular weight
21184 MW
Background of BAX
Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1013-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, By Heat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Testis Tissue, Rat Kidney Tissue, Rat Brain Tissue, Rat Ovary Tissue, HELA Cell, MM231 Cell, A549 Cell, JURKAT Cell, Human Placenta Tissue
IHC: human ovarian cancer tissue
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Bax using anti-Bax antibody (PA1013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: Human Placenta Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.
Click image to see more details
Figure 2. IHC analysis of Bax using anti-Bax antibody (PA1013-1).
Bax was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bax Antibody (PA1013-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of A431 cells using anti-BAX antibody (PA1013-1).
Overlay histogram showing A431 cells stained with PA1013-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAX Antibody (PA1013-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For BAX (Source: Uniprot.org, NCBI)
Gene Name
BAX
Full Name
Apoptosis regulator BAX
Weight
21184 MW
Superfamily
Bcl-2 family
Alternative Names
Apoptosis regulator BAX;Bcl-2-like protein 4;Bcl2-L-4;BAX;BCL2L4; BAX BCL2L4 BCL2 associated X, apoptosis regulator apoptosis regulator BAX|BCL2 associated X protein|BCL2-associated X protein omega|Baxdelta2G9|Baxdelta2G9omega|Baxdelta2omega|bcl-2-like protein 4|bcl2-L-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAX, check out the BAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apoptosis regulator BAX Bax Antibody Picoband® (PA1013-1)
Hello CJ!
PA1013-1 has been cited in 128 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng K,Yuan G,Bao H,Wang L,Ma R,Yu B,Zhao S.Interaction of HCCR-1 and Bax in breast cancer.J BUON.2019 May-Jun;24(3):1027-1037.PMID:31424657.
Species: Human
PA1013-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Su-Su Tang,Yi Ren,Xiao-Qian Ren,Jing-Ran Cao,Hao Hong,Hui Ji,Qing-Hua Hu,ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice,Experimental Neurology,Volume 311,2019,Pages 33-43,ISSN 0014-4886,https://doi.org/10.1016/j.expneurol.2018.09.002.
Species: Mouse
PA1013-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:500
Ren Q,Hu Z,Jiang Y,Tan X,Botchway BOA,Amin N,Lin G,Geng Y,Fang M.SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury.Front Neurol.2019 Dec 5;10:1289.doi:10.3389/fneur.2019.01289.PMID:31920915;PMCID:PMC6915092.
Species: Mouse
PA1013-1 usage in article: APP:WB, SAMPLE: PC12 CELL, DILUTION:1:400
Zhang X,Guo S,Huang X,Li B,Dai H,Wang C.A novel macrolide derivative ameliorates smoke-induced inflammation and emphysema by inhibiting NF-κB activation.Am J Transl Res.2021 Mar 15;13(3):1209-1220.PMID:33841650;PMCID:PMC8014339.
Species: Mouse
PA1013-1 usage in article: APP:IHC, SAMPLE:TRACHEAL TISSUE, DILUTION:1:50
Song P, Yan B, Lei F, Qiu Z, Zhang C, Wu Y, Chen S, Yang X, Shen D, Ma P. Continuous artificial light at night exacerbates diisononyl phthalate-induced learning and memory impairment in mice: Toxicological evidence. Food Chem Toxicol. 2021 Mar 9;151: 112102. doi: 10.1016/j.fct.2021.112102. Epub ahead of print. PMID: 33711377.
Species: Mouse
PA1013-1 usage in article: APP:IHC, SAMPLE: HIPPOCAMPUL TISSUE, DILUTION:NA
Junyi Tang,Kehua Fang,Chang Li,Xiaotian Chang,"ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6683264,11 pages,2021.https://doi.org/10.1155/2021/6683264
Species: Human
PA1013-1 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA
Zhang Z, Liu W, Shen M, Ma X, Li R, Jin X, Bai H, Gao L. Protective Effect of GM1 Attenuates Hippocampus and Cortex Apoptosis After Ketamine Exposure in Neonatal Rat via PI3K/AKT/GSK3β Pathway. Mol Neurobiol. 2021 Mar 17. doi: 10.1007/s12035-021-02346-5.Epub ahead of print.PMID: 33733293.
Species: Rat
PA1013-1 usage in article: APP:IHC, SAMPLE:TUMOR TISSUE, DILUTION:NA
Liang,T.,Wei,C.,Lu,S.,Qin,M.,Qin,G.,Zhang,Y. ... Yang,Y. (2021).Ginaton injection alleviates cisplatin‑induced renal interstitial fibrosis in rats via inhibition of apoptosis through regulation of the p38MAPK/TGF‑β1 and p38MAPK/HIF‑1α pathways.Biomedical
Species: Rat
PA1013-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Xu Y,Chen W,Chen Z,Huang M,Yang F,Zhang Y.Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.Drug Des Devel Ther.2021 Feb 22;15:753-767.doi:10.2147/DDDT.S280217.PMID:33654381;
Species: Rat
PA1013-1 usage in article: APP:WB, SAMPLE:PC12 CELL, DILUTION:1:500
Zhou L,Liu Y,Wang Z,Liu D,Xie B,Zhang Y,Yuan M,Tse G,Li G,Xu G,Liu T.Activation of NADPH oxidase mediates mitochondrial oxidative stress and atrial remodeling in diabetic rabbits.Life Sci.2021 Feb 15:119240.doi:10.1016/j.lfs.2021.119240.Epub ahead of prin
Species: Rabbit
PA1013-1 usage in article: APP:WB, SAMPLE:ATRIAL TISSUE, DILUTION:1:300
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Apoptosis regulator BAX Bax Antibody Picoband®
Question
Is a blocking peptide available for product anti-Bax antibody (PA1013-1)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-Bax antibody (PA1013-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
Is this PA1013-1 anti-Bax antibody reactive to the isotypes of BAX?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The immunogen of PA1013-1 anti-Bax antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Bax(19-33aa IMKTGALLLQGFIQD), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-13
Question
Will PA1013-1 anti-Bax antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
It shows on the product datasheet, PA1013-1 anti-Bax antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
I am interested in using your anti-Bax antibody for post-embryonic camera-type eye morphogenesis studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We appreciate your inquiry. This PA1013-1 anti-Bax antibody is validated on rat ovary tissue, testis tissue, tissue lysate, kidney tissue, brain tissue, hela cell lysate, mm231 cell lysate, a549 cell lysate, jurkat cell lysate, human placenta tissue, a431 cells. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax antibody PA1013-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-16
Question
Can you help my question with product PA1013-1, anti-Bax antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1013-1 anti-Bax antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-19
Question
Does anti-Bax antibody PA1013-1 work on monkey WB with brain?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Our lab technicians have not tested anti-Bax antibody PA1013-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Bax antibody PA1013-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
Is there a BSA free version of anti-Bax antibody PA1013-1 available?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bax antibody PA1013-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-14
Question
We are interested in to test anti-Bax antibody PA1013-1 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-Bax antibody PA1013-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
The products we sell, including anti-Bax antibody PA1013-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-21
Question
We bought anti-Bax antibody for Flow Cytometry on mucosa of transverse colon a few years ago. I am using human, and We intend to use the antibody for WB next. I was wanting to use examining mucosa of transverse colon as well as ovarian carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
P. Dhar
Verified customer
Asked: 2018-06-12
Answer
I looked at the website and datasheets of our anti-Bax antibody and it appears that PA1013-1 has been validated on human in both Flow Cytometry and WB. Thus PA1013-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-12
Question
We have been able to see staining in mouse skin. Do you have any suggestions? Is anti-Bax antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
From literature skin does express BAX. From Uniprot.org, BAX is expressed in mucosa of transverse colon, b-cell, brain, ovarian carcinoma, skin, among other tissues. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-22
Question
My colleagues were content with the WB result of your anti-Bax antibody. However we have seen positive staining in skin isoform beta: cytoplasm. using this antibody. Is that expected? Could you tell me where is BAX supposed to be expressed?
K. Johnson
Verified customer
Asked: 2017-11-21
Answer
According to literature, skin does express BAX. Generally BAX expresses in isoform alpha: mitochondrion outer membrane, isoform beta: cytoplasm., isoform gamma: cytoplasm., isoform delta: cytoplasm. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-11-21
Question
I have attached the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax antibody PA1013-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-22
Question
I was wanting to use your anti-Bax antibody for WB for human mucosa of transverse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mucosa of transverse colon identification?
K. Kulkarni
Verified customer
Asked: 2017-02-16
Answer
You can see on the product datasheet, PA1013-1 anti-Bax antibody has been validated for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-16
Question
I see that the anti-Bax antibody PA1013-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Jackson
Verified customer
Asked: 2016-09-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-09-09
Question
Does anti-Bax antibody PA1013-1 work for WB with mucosa of transverse colon?
G. Krishna
Verified customer
Asked: 2015-06-02
Answer
According to the expression profile of mucosa of transverse colon, BAX is highly expressed in mucosa of transverse colon. So, it is likely that anti-Bax antibody PA1013-1 will work for WB with mucosa of transverse colon.
Boster Scientific Support
Answered: 2015-06-02
Question
We are currently using anti-Bax antibody PA1013-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
D. Carter
Verified customer
Asked: 2015-02-02
Answer
The anti-Bax antibody (PA1013-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-02
