Anti-PPID Antibody

Product Name

Anti-PPID Antibody

View all Cyclophilin 40 Antibodies

SKU/Catalog Number

PA1472

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPID Antibody catalog # PA1472. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPID Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1472)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PPID, different from the related mouse and rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1472 is reactive to PPID in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

40764 MW

Background of Cyclophilin 40

Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. The Cyclophilin D (PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1472 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PPID using anti-PPID antibody (PA1472).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: human THP-1 whole cell lysates,
Lane 8: rat liver tissue lysates.
Lane 9: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPID antigen affinity purified polyclonal antibody (Catalog # PA1472) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPID at approximately 38 kDa. The expected band size for PPID is at 41 kDa.

Click image to see more details

Figure 2. IHC analysis of PPID using anti-PPID antibody (PA1472).
PPID was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPID Antibody (PA1472) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For PPID (Source: Uniprot.org, NCBI)

Alternative Names

40 kDa peptidyl-prolyl cis-trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; cyclophilin D; cyclophilin-40; Cyclophilin-related protein; CYP40; CYP-40cyclophilin 40; CYPD; EC 5.2.1.8; MGC33096; peptidyl-prolyl cis-trans isomerase D; peptidylprolyl isomerase D (cyclophilin D); peptidylprolyl isomerase D; PPIase D; Rotamase D PPID CYP-40, CYPD peptidylprolyl isomerase D peptidyl-prolyl cis-trans isomerase D|40 kDa peptidyl-prolyl cis-trans isomerase D|PPIase D|cyclophilin 40|cyclophilin D|cyclophilin-related protein|rotamase D|testicular tissue protein Li 147

For more info on PPID, check out the PPID Infographic

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In this infographic, you will see the following information for PPID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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