Product Info Summary
SKU: | FEK0337 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit
See all Fas Ligand/TNFSF6 products
SKU/Catalog Number
FEK0337
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FASL / CD95LG / CD95 Ligand PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0337)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: P134-L281
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0337 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0337 is reactive to FASLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
FEK0337 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fas Ligand/TNFSF6
Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0337-CAP | 96-well plate precoated with anti-Human Fas Ligand/TNFSF6 antibody | 1 |
EK0337-ST | lyophilized recombinant Human Fas Ligand/TNFSF6 standard | 10 ng/tube |
FEK0337-DA | Biotinylated anti-Human Fas Ligand/TNFSF6 detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.008 | 0.114 | 0.196 | 0.379 | 0.740 | 1.371 | 2.121 | 2.693 |
Data Example Images
Click image to see more details
Human FASL PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 36 | 186 | 437 | 34 | 189 | 419 |
Standard deviation | 2.19 | 9.67 | 28.84 | 2.1 | 12.47 | 33.1 |
CV (%) | 6.1% | 5.2% | 6.6% | 6.2% | 6.6% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Fas Ligand/TNFSF6 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 36 | 41 | 41 | 34 | 38 | 3.08 | 8.1% |
Sample 2 | 186 | 197 | 192 | 198 | 193 | 4.76 | 2.4% |
Sample 3 | 437 | 495 | 446 | 507 | 471 | 30.21 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For FASLG (Source: Uniprot.Org, NCBI)
Uniprot ID
P48023
Gene ID
356
Gene Name
FASLG
Full Name
Tumor necrosis factor ligand superfamily member 6
Weight
31485 MW
Superfamily
tumor necrosis factor family
Alternative Names
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6 FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) antigen ligand 1|apoptosis antigen ligand|fas antigen ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FASLG, check out the FASLG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FASLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit (FEK0337)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit
Question
An inquiry: For this antibody FEK0337 at where the FasL protein do the antibodies bind? I want to measure the amount of a chimeric FasL molecule I have made versus the WT FasL protein. The chimeric protein contains AA 139-281 of human trans-membrane FasL. It also has an HA tag so I could just use an antibody against the HA tag if necessary. Do you have any other ELISA kit that I will use against HA tag?
Verified Customer
Verified customer
Asked: 2013-08-12
Answer
The epitopes of this FEK0337 antibody is not known, however, the antibody pair is binding somewhere on the extracellular domain (aa 134-281). The capture antibody is monoclonal and the detection antibody is polyclonal. As for HA tag we do not have a kit against HA though we have many antibodies that are anti-HA.
Boster Scientific Support
Answered: 2013-08-12