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Product Info Summary
| SKU: | M00183-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Bax Rabbit Monoclonal Antibody
SKU/Catalog Number
M00183-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bax Rabbit Monoclonal Antibody catalog # M00183-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bax Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00183-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAB-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bax
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00183-1 is reactive to BAX in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
21 kDa
Calculated molecular weight
21184 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00183-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human THP-1 whole cell, human MCF-7 whole cell, human A549 whole cell, human Hela whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
IHC: Human lung adenocarcinoma tissue, Human Hodgkin's lymphoma tissue, human adenocarcinoma of colon tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Bax using anti-Bax antibody (M00183-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # M00183-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21 kDa. The expected band size for Bax is at 21 kDa.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody.
Protein Target Info & Infographic
Gene/Protein Information For BAX (Source: Uniprot.org, NCBI)
Gene Name
BAX
Full Name
Apoptosis regulator BAX
Weight
21184 MW
Superfamily
Bcl-2 family
Alternative Names
Apoptosis regulator BAX;Bcl-2-like protein 4;Bcl2-L-4;BAX;BCL2L4; BAX BCL2L4 BCL2 associated X, apoptosis regulator apoptosis regulator BAX|BCL2 associated X protein|BCL2-associated X protein omega|Baxdelta2G9|Baxdelta2G9omega|Baxdelta2omega|bcl-2-like protein 4|bcl2-L-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAX, check out the BAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bax Rabbit Monoclonal Antibody (M00183-1)
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Customer Q&As
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16 Customer Q&As for Anti-Bax Rabbit Monoclonal Antibody
Question
We are currently using anti-Bax Rabbit Monoclonal antibody M00183-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-Bax Rabbit Monoclonal antibody (M00183-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
Do you have a BSA free version of anti-Bax Rabbit Monoclonal antibody M00183-1 available?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bax Rabbit Monoclonal antibody M00183-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-01
Question
Please see the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax Rabbit Monoclonal antibody M00183-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Will anti-Bax Rabbit Monoclonal antibody M00183-1 work for IP with mucosa of transverse colon?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
According to the expression profile of mucosa of transverse colon, BAX is highly expressed in mucosa of transverse colon. So, it is likely that anti-Bax Rabbit Monoclonal antibody M00183-1 will work for IP with mucosa of transverse colon.
Boster Scientific Support
Answered: 2020-02-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax Rabbit Monoclonal antibody M00183-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Our lab used your anti-Bax Rabbit Monoclonal antibody for IHC on mucosa of transverse colon a few months ago. I am using mouse, and We intend to use the antibody for IP next. I was wanting to use examining mucosa of transverse colon as well as b-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
I took a look at the website and datasheets of our anti-Bax Rabbit Monoclonal antibody and I see that M00183-1 has been tested on mouse in both IHC and IP. Thus M00183-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-Bax Rabbit Monoclonal antibody for IP for human mucosa of transverse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mucosa of transverse colon identification?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, M00183-1 anti-Bax Rabbit Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this M00183-1 anti-Bax Rabbit Monoclonal antibody reactive to the isotypes of BAX?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The immunogen of M00183-1 anti-Bax Rabbit Monoclonal antibody is A synthesized peptide derived from human Bax. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-26
Question
Would M00183-1 anti-Bax Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
As indicated on the product datasheet, M00183-1 anti-Bax Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-19
Question
My question regarding product M00183-1, anti-Bax Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Krishna
Verified customer
Asked: 2018-08-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00183-1 anti-Bax Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-27
Question
I see that the anti-Bax Rabbit Monoclonal antibody M00183-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-24
Question
I am interested in using your anti-Bax Rabbit Monoclonal antibody for b cell negative selection studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
We appreciate your inquiry. This M00183-1 anti-Bax Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-21
Question
We need to test anti-Bax Rabbit Monoclonal antibody M00183-1 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-Bax Rabbit Monoclonal antibody M00183-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
The products we sell, including anti-Bax Rabbit Monoclonal antibody M00183-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-12
Question
We have seen staining in mouse mucosa of transverse colon. Are there any suggestions? Is anti-Bax Rabbit Monoclonal antibody supposed to stain mucosa of transverse colon positively?
K. Krishna
Verified customer
Asked: 2016-12-21
Answer
From literature mucosa of transverse colon does express BAX. From Uniprot.org, BAX is expressed in mucosa of transverse colon, b-cell, brain, ovarian carcinoma, skin, among other tissues. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-12-21
Question
My colleagues were well pleased with the WB result of your anti-Bax Rabbit Monoclonal antibody. However we have seen positive staining in b-cell isoform beta: cytoplasm. using this antibody. Is that expected? Could you tell me where is BAX supposed to be expressed?
C. Mitchell
Verified customer
Asked: 2015-05-14
Answer
From what I have seen in literature, b-cell does express BAX. Generally BAX expresses in isoform alpha: mitochondrion outer membrane, isoform beta: cytoplasm., isoform gamma: cytoplasm., isoform delta: cytoplasm. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-05-14
Question
Is a blocking peptide available for product anti-Bax Rabbit Monoclonal antibody (M00183-1)?
H. Brown
Verified customer
Asked: 2014-03-19
Answer
We do provide the blocking peptide for product anti-Bax Rabbit Monoclonal antibody (M00183-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-19
