Click image to see more details
Product Info Summary
| SKU: | PB10010 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-B7 DC/PDCD1LG2 Antibody Picoband®
View all PD-L2/B7-DC/PDCD1LG2 Antibodies
SKU/Catalog Number
PB10010
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-B7 DC/PDCD1LG2 Antibody Picoband® catalog # PB10010. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B7 DC/PDCD1LG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10010)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human B7 DC recombinant protein (Position: L20-T220). Human B7 DC shares 72.1% amino acid (aa) sequence identity with mouse B7 DC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10010 is reactive to PDCD1LG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
30957 MW
Background of PD-L2/B7-DC/PDCD1LG2
Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10010 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PDCD1LG2 (Source: Uniprot.org, NCBI)
Gene Name
PDCD1LG2
Full Name
Programmed cell death 1 ligand 2
Weight
30957 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Programmed cell death 1 ligand 2;PD-1 ligand 2;PD-L2;PDCD1 ligand 2;Programmed death ligand 2;Butyrophilin B7-DC;B7-DC;CD273;PDCD1LG2;B7DC, CD273, PDCD1L2, PDL2; Pdcd1lg2|B7-DC, Btdc, F730015O22Rik, PD-, PD-L2|programmed cell death 1 ligand 2|programmed cell death 1 ligand 2|PD-1 ligand 2|PDCD1 ligand 2|butyrophilin B7-DC|butyrophilin-like protein|programmed death ligand 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD1LG2, check out the PDCD1LG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD1LG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B7 DC/PDCD1LG2 Antibody Picoband® (PB10010)
Hello CJ!
No publications found for PB10010
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-B7 DC/PDCD1LG2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-B7 DC/PDCD1LG2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-B7 DC/PDCD1LG2 Antibody Picoband®
Question
My question regarding product PB10010, anti-B7 DC/PDCD1LG2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10010 anti-B7 DC/PDCD1LG2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-B7 DC/PDCD1LG2 antibody PB10010 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-B7 DC/PDCD1LG2 antibody (PB10010) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
Would PB10010 anti-B7 DC/PDCD1LG2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Mangal
Verified customer
Asked: 2019-07-11
Answer
It shows on the product datasheet, PB10010 anti-B7 DC/PDCD1LG2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-11
Question
Do you have a BSA free version of anti-B7 DC/PDCD1LG2 antibody PB10010 available?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-B7 DC/PDCD1LG2 antibody PB10010 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-10
Question
I see that the anti-B7 DC/PDCD1LG2 antibody PB10010 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-24
Question
Is this PB10010 anti-B7 DC/PDCD1LG2 antibody reactive to the isotypes of PDCD1LG2?
J. Yang
Verified customer
Asked: 2013-04-23
Answer
The immunogen of PB10010 anti-B7 DC/PDCD1LG2 antibody is E.coli-derived human B7 DC recombinant protein (Position: L20-T220). Human B7 DC shares 72.1% amino acid (aa) sequence identity with mouse B7 DC. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-04-23
