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Product Info Summary
| SKU: | M09565 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATP5H in Antibody Picoband® (monoclonal, 6B12)
SKU/Catalog Number
M09565
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATP5H in Antibody Picoband® (monoclonal, 6B12) catalog # M09565. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5H in Antibody Picoband® (monoclonal, 6B12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09565)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6B12
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M09565 is reactive to ATP5H in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
18.491kDa
Background of ATP5PD
ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09565 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human Hela tissue, human HepG2 whole cell, human U-87MG whole cell, human A549 whole cell, monkey COS-7 whole cell, human SW620 whole cellrat PC-12 whole cellmouse Ana-1 whole cell
IHC: human B lymphocytic tumorT tissue, human mammary cancer tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (M09565).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: monkey COS-7 whole cell lysates,
Lane 6: human SW620 whole cell lysates.
Lane 7: rat PC-12 whole cell lysates.
Lane 8: mouse Ana-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ATP5H antigen affinity purified polyclonal antibody (Catalog # M09565) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22KD. The expected band size for ATP5H is at 18KD.
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Figure 2. IHC analysis of ATP5H using anti-ATP5H antibody (M09565).
ATP5H was detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 3. IHC analysis of ATP5H using anti-ATP5H antibody (M09565).
ATP5H was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 4. IF analysis of ATP5H using anti-ATP5H antibody (M09565).
ATP5H was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ATP5H (Source: Uniprot.org, NCBI)
Gene Name
ATP5H
Full Name
ATP synthase subunit d, mitochondrial
Weight
18.491kDa
Superfamily
ATPase d subunit family
Alternative Names
ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody ATP5PD APT5H, ATP5H, ATPQ ATP synthase peripheral stalk subunit d ATP synthase subunit d, mitochondrial|ATP synthase D chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d|ATP synthase, H+ transporting, mitochondrial F1F0, subunit d|ATP synthase, H+ transporting, mitochondrial Fo complex subunit D|ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d|ATPase subunit d|My032 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5H, check out the ATP5H Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5H in Antibody Picoband® (monoclonal, 6B12) (M09565)
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