Anti-Zinc finger protein 521 ZNF521 Antibody

Product Name

Anti-Zinc finger protein 521 ZNF521 Antibody

View all ZNF521 Antibodies

SKU/Catalog Number

A06292

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Zinc finger protein 521 ZNF521 Antibody (Catalog # A06292). Tested in ELISA, IHC-P applications. This antibody reacts with Human.

Storage & Handling

ZNF521 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Zinc finger protein 521 ZNF521 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06292)

Host

Rabbit

Contents

ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZNF521 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZNF521. The immunogen is located within amino acids 1160 - 1210 of ZNF521.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ZNF521 antibody is human specific.

Reactive Species

A06292 is reactive to ZNF521 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

147866 MW

Background of ZNF521

The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06292 is guaranteed for ELISA, IHC-P Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZNF521 antibody can be used for detection of ZNF521 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ZNF521 (Source: Uniprot.org, NCBI)

Alternative Names

EHZF, Evi3, EHZF, LIP3, Zinc finger protein 521, Early hematopoietic zinc finger protein ZNF521 EHZF, Evi3 zinc finger protein 521 zinc finger protein 521|LYST-interacting protein 3|early hematopoietic zinc finger protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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