Smad2 (Phospho-Ser467) Colorimetric Cell-Based ELISA Kit

Smad2 ELISA kit, phospho-specific, cell-based

The Smad2 (Phospho-Ser467) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad2 (Phospho-Ser467) protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad2 (Phospho-Ser467) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad2 (Phospho-Ser467).

Product Info Summary

SKU: EKC1959
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

Smad2 (Phospho-Ser467) Colorimetric Cell-Based ELISA Kit

See all Smad2 products

SKU/Catalog Number

EKC1959

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The Smad2 (Phospho-Ser467) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad2 (Phospho-Ser467) protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad2 (Phospho-Ser467) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad2 (Phospho-Ser467).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Smad2 (Phospho-Ser467) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1959)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1959 is reactive to SMAD2 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1959 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Smad2 Phospho-Ser467 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-Smad2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For SMAD2 (Source: Uniprot.Org, NCBI)

Gene Name

SMAD2

Full Name

Mothers against decapentaplegic homolog 2

Weight

52306 MW

Superfamily

dwarfin/SMAD family

Alternative Names

JV18-1;MADH2;MADR2;Mad-related protein 2;Mothers against DPP homolog 2;Mothers against decapentaplegic homolog 2;Smad 2 SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SMAD2, check out the SMAD2 Infographic

SMAD2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SMAD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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