Click image to see more details
Product Info Summary
| SKU: | M00090-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Smad2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00090-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Smad2 Rabbit Monoclonal Antibody catalog # M00090-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00090-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00090-1 is reactive to SMAD2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
52306 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00090-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human MCF-7 whole cell, human MDA-MB-453 whole cell, rat C6 whole cell, mouse RAW2647 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (M00090-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human MDA-MB-453 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified monoclonal antibody (Catalog # M00090-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 52 kDa. The expected band size for SMAD2 is at 52 kDa.
Click image to see more details
Immunofluorescent analysis of Hela cells, using Smad2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMAD2 (Source: Uniprot.org, NCBI)
Gene Name
SMAD2
Full Name
Mothers against decapentaplegic homolog 2
Weight
52306 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad2 Rabbit Monoclonal Antibody (M00090-1)
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Customer Q&As
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16 Customer Q&As for Anti-Smad2 Rabbit Monoclonal Antibody
Question
My question regarding product M00090-1, anti-Smad2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00090-1 anti-Smad2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-30
Question
I see that the anti-Smad2 Rabbit Monoclonal antibody M00090-1 works with IF, what is the protocol used to produce the result images on the product page?
A. Singh
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-21
Question
I was wanting to use your anti-Smad2 Rabbit Monoclonal antibody for IF for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, M00090-1 anti-Smad2 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Smad2 Rabbit Monoclonal antibody M00090-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-Smad2 Rabbit Monoclonal antibody M00090-1 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
The anti-Smad2 Rabbit Monoclonal antibody (M00090-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-10
Question
My lab would like using your anti-Smad2 Rabbit Monoclonal antibody for in utero embryonic development studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
I appreciate your inquiry. This M00090-1 anti-Smad2 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-26
Question
Will anti-Smad2 Rabbit Monoclonal antibody M00090-1 work for IF with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
According to the expression profile of cervix carcinoma erythroleukemia, SMAD2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Smad2 Rabbit Monoclonal antibody M00090-1 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-11-02
Question
We have seen staining in human pancreas spleen. Do you have any suggestions? Is anti-Smad2 Rabbit Monoclonal antibody supposed to stain pancreas spleen positively?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
According to literature pancreas spleen does express SMAD2. According to Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-09-17
Question
Is there a BSA free version of anti-Smad2 Rabbit Monoclonal antibody M00090-1 available?
E. Mitchell
Verified customer
Asked: 2018-04-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Smad2 Rabbit Monoclonal antibody M00090-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-10
Question
Is this M00090-1 anti-Smad2 Rabbit Monoclonal antibody reactive to the isotypes of SMAD2?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The immunogen of M00090-1 anti-Smad2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Smad2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-26
Question
My team were satisfied with the WB result of your anti-Smad2 Rabbit Monoclonal antibody. However we have been able to see positive staining in colon adenocarcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?
G. Bhatt
Verified customer
Asked: 2018-01-17
Answer
From what I have seen in literature, colon adenocarcinoma does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-01-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Smad2 Rabbit Monoclonal antibody M00090-1. Let me know if you need anything else.
W. Krishna
Verified customer
Asked: 2017-12-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-21
Question
My lab would like to test anti-Smad2 Rabbit Monoclonal antibody M00090-1 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Smad2 Rabbit Monoclonal antibody M00090-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
The products we sell, including anti-Smad2 Rabbit Monoclonal antibody M00090-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-22
Question
Is a blocking peptide available for product anti-Smad2 Rabbit Monoclonal antibody (M00090-1)?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
We do provide the blocking peptide for product anti-Smad2 Rabbit Monoclonal antibody (M00090-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-17
Question
Will M00090-1 anti-Smad2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Taylor
Verified customer
Asked: 2014-09-03
Answer
As indicated on the product datasheet, M00090-1 anti-Smad2 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-03
Question
We ordered your anti-Smad2 Rabbit Monoclonal antibody for WB on cervix carcinoma erythroleukemia in the past. I am using mouse, and We are going to use the antibody for IF next. I am interested in examining cervix carcinoma erythroleukemia as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Patel
Verified customer
Asked: 2014-02-28
Answer
I have checked the website and datasheets of our anti-Smad2 Rabbit Monoclonal antibody and I see that M00090-1 has been validated on mouse in both WB and IF. Thus M00090-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-02-28
