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Product Info Summary
| SKU: | PA1898 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Smad2 Antibody Picoband®
SKU/Catalog Number
PA1898
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smad2 Antibody catalog # PA1898. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1898)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1898 is reactive to SMAD2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
52306 MW
Background of Smad2
Smad2 (Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. Eppert et al. (1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al. (1996) mapped the human MADH2 gene to 18q21. Nakao et al. (1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1898 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry , 0.5-1μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human MDA-MB-453 whole cell
IHC: human mammary cancer tissue
ICC: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (PA1898).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human MDA-MB-453 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # PA1898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60 kDa. The expected band size for SMAD2 is at 60 kDa.
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Figure 2. IHC analysis of SMAD2 using anti-SMAD2 antibody (PA1898).
SMAD2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMAD2 Antibody (PA1898) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. ICC analysis of SMAD2 using anti-SMAD2 antibody (PA1898).
SMAD2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4 μg/ml rabbit anti-SMAD2 Antibody (PA1898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SMAD2 (Source: Uniprot.org, NCBI)
Gene Name
SMAD2
Full Name
Mothers against decapentaplegic homolog 2
Weight
52306 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad2 Antibody Picoband® (PA1898)
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Customer Q&As
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17 Customer Q&As for Anti-Smad2 Antibody Picoband®
Question
Is this PA1898 anti-Smad2 antibody reactive to the isotypes of SMAD2?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The immunogen of PA1898 anti-Smad2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-13
Question
We are interested in using your anti-Smad2 antibody for signal transduction involved in regulation of gene expression studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thank you for your inquiry. This PA1898 anti-Smad2 antibody is tested on mammary cancer tissue, tissue lysate, rat brain tissue, hela cell lysate, smmc cell lysate, jurkat cell lysate, u87 cell lysate. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-06
Question
Will anti-Smad2 antibody PA1898 work on monkey WB with cervix carcinoma?
A. Miller
Verified customer
Asked: 2020-03-12
Answer
Our lab technicians have not tested anti-Smad2 antibody PA1898 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Smad2 antibody PA1898 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is a blocking peptide available for product anti-Smad2 antibody (PA1898)?
J. Krishna
Verified customer
Asked: 2020-01-06
Answer
We do provide the blocking peptide for product anti-Smad2 antibody (PA1898). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-06
Question
Please see the WB image, lot number and protocol we used for kidney using anti-Smad2 antibody PA1898. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
We bought anti-Smad2 antibody for WB on kidney last year. I am using mouse, and We intend to use the antibody for ICC next. I was wanting to use examining kidney as well as colon adenocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
I viewed the website and datasheets of our anti-Smad2 antibody and I see that PA1898 has been tested on mouse in both WB and ICC. Thus PA1898 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-17
Question
I am interested in to test anti-Smad2 antibody PA1898 on human kidney for research purposes, then I may be interested in using anti-Smad2 antibody PA1898 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The products we sell, including anti-Smad2 antibody PA1898, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-13
Question
I see that the anti-Smad2 antibody PA1898 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
Can you help my question with product PA1898, anti-Smad2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1898 anti-Smad2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
Will anti-Smad2 antibody PA1898 work for WB with kidney?
J. Johnson
Verified customer
Asked: 2019-07-03
Answer
According to the expression profile of kidney, SMAD2 is highly expressed in kidney. So, it is likely that anti-Smad2 antibody PA1898 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-07-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Smad2 antibody PA1898. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-24
Question
We have seen staining in mouse pancreas spleen. Do you have any suggestions? Is anti-Smad2 antibody supposed to stain pancreas spleen positively?
S. Collins
Verified customer
Asked: 2018-11-26
Answer
According to literature pancreas spleen does express SMAD2. According to Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-11-26
Question
I was wanting to use your anti-Smad2 antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
As indicated on the product datasheet, PA1898 anti-Smad2 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-10
Question
Do you have a BSA free version of anti-Smad2 antibody PA1898 available?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Smad2 antibody PA1898 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-01
Question
We are currently using anti-Smad2 antibody PA1898 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-Smad2 antibody (PA1898) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
Question
My colleagues were content with the WB result of your anti-Smad2 antibody. However we have observed positive staining in colon adenocarcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?
J. Johnson
Verified customer
Asked: 2014-12-25
Answer
Based on literature, colon adenocarcinoma does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2014-12-25
Question
Does PA1898 anti-Smad2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Edwards
Verified customer
Asked: 2013-09-18
Answer
You can see on the product datasheet, PA1898 anti-Smad2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-18
