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Product Info Summary
| SKU: | RP1102 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SMURF 2/SMURF2 Antibody Picoband®
SKU/Catalog Number
RP1102
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMURF 2/SMURF2 Antibody catalog # RP1102. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMURF 2/SMURF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1102)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SMURF 2, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
RP1102 is reactive to SMURF2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
86 kDa
Calculated molecular weight
86196 MW
Background of SMURF2
E3 ubiquitin-protein ligase SMURF2 is an enzyme that in humans is encoded by the SMURF2 gene. The SMURF2 gene is mapped to chromosome 17q22-q23 based on sequence similarity between the SMURF2 sequence and a genomic contig. SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs, TGF-beta receptor (TGFBR), and other substrates. It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1102 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: SMMC whole cell, rat testis tissue, rat pancreas tissue, rat stomach testis, mouse testis tissuemouse pancreas tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SMURF2 using anti-SMURF2 antibody (RP1102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SMMC whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # RP1102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.
Click image to see more details
Figure 2. Western blot analysis of SMURF2 using anti-SMURF2 antibody (RP1102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: rat stomach testis lysates,
Lane 4: mouse testis tissue lysates.
Lane 5: mouse pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # RP1102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.
Protein Target Info & Infographic
Gene/Protein Information For SMURF2 (Source: Uniprot.org, NCBI)
Gene Name
SMURF2
Full Name
E3 ubiquitin-protein ligase SMURF2
Weight
86196 MW
Alternative Names
E3 ubiquitin-protein ligase SMURF2;hSMURF2;6.3.2.-;SMAD ubiquitination regulatory factor 2;SMAD-specific E3 ubiquitin-protein ligase 2;SMURF2; SMURF2 SMAD specific E3 ubiquitin protein ligase 2 E3 ubiquitin-protein ligase SMURF2|E3 ubiquitin ligase SMURF2|HECT-type E3 ubiquitin transferase SMURF2|SMAD ubiquitination regulatory factor 2|hSMURF2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMURF2, check out the SMURF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMURF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMURF 2/SMURF2 Antibody Picoband® (RP1102)
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1 Customer Q&As for Anti-SMURF 2/SMURF2 Antibody Picoband®
Question
We are currently using anti-SMURF 2/SMURF2 antibody RP1102 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
K. Jones
Verified customer
Asked: 2017-09-25
Answer
The anti-SMURF 2/SMURF2 antibody (RP1102) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-25
