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Product Info Summary
| SKU: | EK0639 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Rat MMP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0639
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat MMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat MMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0639)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A30-C662
Sensitivity
<10 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0639 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0639 is reactive to Mmp2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0639 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-2
Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0639-CAP | Anti-Rat Mmp2 Pre-coated 96-well strip microplate | 1 |
| EK0639-ST | Rat Mmp2 Standard | 2 vials, 40 ng/tube |
| EK0639-DA | Rat Mmp2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.131 | 0.168 | 0.213 | 0.332 | 0.585 | 0.925 | 1.498 | 2.082 |
Data Example Images
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Rat MMP-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-2, Dilution ratio of 1:10, concentration in serum is 45-125 ng/ml..
Some articles we found to cite concentrations of MMP-2 in samples: 24033225 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat MMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1093 | 4492 | 23073 | 1098 | 4440 | 21240 |
| Standard deviation | 68.85 | 296.47 | 1107.5 | 90.03 | 368.52 | 1296.64 |
| CV (%) | 8.3% | 6.6% | 4.8% | 8.2% | 8.3% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1093 | 1090 | 975 | 1117 | 1068 | 55.12 | 5.1% |
| Sample 2 | 4492 | 3849 | 4402 | 4495 | 4309 | 268.48 | 6.2% |
| Sample 3 | 23073 | 23113 | 22919 | 26456 | 23890 | 1483.1 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)
Gene Name
Mmp2
Full Name
72 kDa type IV collagenase
Weight
74149 MW
Superfamily
peptidase M10A family
Alternative Names
72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;PEX;Mmp2; MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Mmp2, check out the Mmp2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat MMP-2 ELISA Kit PicoKine® (EK0639)
Hello CJ!
EK0639 has been cited in 33 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts
KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin
Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus
A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats
Aspirin Ameliorates Preeclampsia Induced by a Peroxisome Proliferator-Activated Receptor Antagonist:
Effects of Curcumin Nanoparticles in Isoproterenol-Induced Myocardial Infarction
Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymosan‐induced synovial inflammation
Effects of Renin‐Angiotensin‐Aldosterone System Blockade on Chlorhexidine Gluconate‐Induced Sclerosing Encapsulated Peritonitis in Rats
Attenuation of lipid metabolic abnormalities, proinflammatory cytokines, and matrix metalloproteinase expression by biochanin-A in isoproterenol-induced myocardial infarction in rats
Effect of crocetin on vascular smooth muscle cells migration induced by advanced glycosylation end products
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Rat MMP-2 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have obtained MMP-2 level in serum.
A. Wang
Verified customer
Asked: 2019-05-27
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-27
Question
Q: can I use EDTA plasma as samples in Rat MMP-2 Picokine® ELISA Kit (Catalog # EK0639)?
Verified Customer
Verified customer
Asked: 2019-05-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MMP-2 causing degradation of its protein structure. MMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-2 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MMP-2 using the EK0639 Rat MMP-2 Picokine® ELISA Kit?
G. Anderson
Verified customer
Asked: 2018-12-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-12-14
Question
Q: which procedure should I follow in order to thaw whole blood sample for MMP-2 ELISA after freezing?
W. Tiwari
Verified customer
Asked: 2018-12-11
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-11
Question
Q: can you recommend the dilution ratio of serum samples for detection of MMP-2 in Rat serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-01
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-01
Question
Q: Can MMP-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Banerjee
Verified customer
Asked: 2018-05-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: What is the optimal O.D. value for MMP-2 ELISA kit? I used your MMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MMP-2 even though the O.D. values are not very high?
M. Johnson
Verified customer
Asked: 2018-04-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-04-19
Question
Q: if the enzyme conjugated MMP-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-2 antigen?
Verified Customer
Verified customer
Asked: 2016-11-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-11-14
Question
Does EK0639 detect active MMP-2 or the full MMP-2 rat protein? Will it cross-react with other proteins?
Verified Customer
Verified customer
Asked: 2016-09-12
Answer
EK0639 is able to detect the full MMP-2 rat protein including zymogen and active MMP-2. It cross reacts with recombinant human MMP-2. Recombinant mouse MMP-3 protein, mouse MMP-12 protein and rat MMP-9 protein was also tested and no significant cross reactivity was observed.
Boster Scientific Support
Answered: 2016-09-12
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-09-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-09-05
