Rat MMP-2 PicoKine® Quick ELISA Kit

Product Name

Rat MMP-2 PicoKine® Quick ELISA Kit

See all MMP-2 products

SKU/Catalog Number

FEK0639

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat MMP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Rat MMP-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0639)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A30-C662

Sensitivity

<300 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0639 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0639 is reactive to Mmp2 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK0639 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMP-2

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0639-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0639-ST	Rat MMP-2 Standard	2	40 ng/tube
FEK0639-A	Rat Mmp2 Antibody A	1	3ml
FEK0639-B	Rat Mmp2 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.029	0.115	0.173	0.310	0.537	0.976	1.555	2.153

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat MMP-2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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