Human VCAM-1 PicoKine™ ELISA Kit

SKU EK0537
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100ul per well
Sensitivity <10pg/ml
Assay Range 156pg/ml-10000pg/ml

Overview

Product Name Human VCAM-1 PicoKine™ ELISA Kit
SKU/Catalog Number EK0537
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VCAM-1. 96wells/kit, with removable strips.
Cite This Product Human VCAM-1 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0537)
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). . Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <10pg/ml
Assay Range 156pg/ml-10000pg/ml
Immunogen Expression system for standard:NSO; Immunogen sequence:F25 - E698
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
EK0537-CAP 96-well plate precoated with anti-Human VCAM1 antibody 1
EK0537-ST lyophilized recombinant Human VCAM1 standard 10ng/tube
EK0537-DA biotinylated anti-Human VCAM1 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human VCAM-1 PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-25min)

Concentration(pg/ml)015631262512502500500010000
O.D.0.0330.1570.2450.4750.8441.2931.7352.365

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3101065528730410295603
Standard deviation17.6751.12387.3519.1565.85481.85
CV(%)5.7%4.8%7.3%6.4%6.4%8.6%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131027329627328815.795.4%
Sample 2106511111014939103263.846.1%
Sample 352875539588460735695303.895.3%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P19320
Gene Name VCAM1
Protein Name Vascular cell adhesion protein 1
Tissue Specificity Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Alternative Names Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100;CD106;VCAM1;L1CAM;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 81276 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Research Areas Human

*You can search these to find other products in these research areas.
Background Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Detect Human VCAM1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. VCAM1 tissue specificity: Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
EK0537
Limited time multi-pack pricing available only to end users in US and Canada.
$369.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for EK0537

Publications

Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Catalpol ameliorates diabetic atherosclerosis in diabetic rabbits
Catalpol ameliorates diabetic atherosclerosis in diabetic rabbits
Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells
Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer
Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X
Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial
Neurally mediated syncope: Is it really an endothelial dysfunction?
Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults
FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness
Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, Lin W, Zhang F. Oxid Med Cell Longev. 2017;2017:6905217. doi: 10.1155/2017/6905217. Epub 2017 Nov 19. Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation o...
Maggi P, Bruno G, Perilli F, Saracino A, Volpe A, Santoro C, Ladisa N, Angarano G. In Vivo. 2017 Jan 2;31(1):125-131. Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients
Denkovskij, J., Bagdonas, E., Kusleviciute, I., Mackiewicz, Z., Unguryte, A., Porvaneckas, N.,..., & Bernotiene, E. (2017). Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases an...
Sun H, Zhu X, Cai W, Qiu L. Sun H1, Zhu X2, Cai W3, Qiu L4. Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway.
Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.
Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.
Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...
Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....
Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?
Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.

Customer Q&As

Copyright © 2019 Boster Biological Technology Inc. All rights reserved.