Click image to see more details
Product Info Summary
| SKU: | A00286 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MMP2 Antibody Picoband®
SKU/Catalog Number
A00286
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP2 Antibody Picoband® catalog # A00286. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00286)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00286 is reactive to MMP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
73882 MW
Background of MMP-2
Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase and is a member of a group of secreted zinc metalloproteases. The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp, located within a region of human chromosome 16q13. In addition, the extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase. MMP2, which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels. Not only is a likely effector of endometrial menstrual breakdown, MMP2 is also effector and regulator of the inflammatory response. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00286 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (A00286).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human MMP2 protein 1ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 71KD. The expected band size for MMP2 is at 71KD.
Protein Target Info & Infographic
Gene/Protein Information For MMP2 (Source: Uniprot.org, NCBI)
Gene Name
MMP2
Full Name
72 kDa type IV collagenase
Weight
73882 MW
Superfamily
peptidase M10A family
Alternative Names
72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;TBE-1;PEX;MMP2;CLG4A; MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP2, check out the MMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP2 Antibody Picoband® (A00286)
Hello CJ!
A00286 has been cited in 46 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Combination of ferulic acid, ligustrazine and tetrahydropalmatine inhibits invasion and metastasis through MMP/TIMP signaling in endometriosis
Mechanism of transmembrane and coiled‑coil domain 1 in the regulation of proliferation and migration of A549 cells
MicroRNA‑153 attenuates hypoxia‑induced excessive proliferation and migration of pulmonary arterial smooth muscle cells by targeting ROCK1 and NFATc3
Exogenous H2S mitigates myocardial fibrosis in diabetic rats through suppression of the canonical Wnt pathway
Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer
Nicotine Alters Morphology and Function of Retinal Pigment Epithelial Cells in Mice:
c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma
Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy
Inhibition effect of Guizhi-Fuling-decoction on the invasion of human cervical cancer
IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MMP2 Antibody Picoband®
Question
Will A00286 anti-MMP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
It shows on the product datasheet, A00286 anti-MMP2 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
Does anti-MMP2 antibody A00286 work for ELISA with melanoma?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of melanoma, MMP2 is highly expressed in melanoma. So, it is likely that anti-MMP2 antibody A00286 will work for ELISA with melanoma.
Boster Scientific Support
Answered: 2020-01-03
Question
I have attached the WB image, lot number and protocol we used for melanoma using anti-MMP2 antibody A00286. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-07
Question
We ordered your anti-MMP2 antibody for ELISA on mucosa of stomach last year. I am using rat, and I plan to use the antibody for WB next. I would like examining mucosa of stomach as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
I viewed the website and datasheets of our anti-MMP2 antibody and it appears that A00286 has been validated on rat in both ELISA and WB. Thus A00286 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-MMP2 antibody A00286. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
I see that the anti-MMP2 antibody A00286 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-05
Question
We are currently using anti-MMP2 antibody A00286 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-MMP2 antibody (A00286) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
We have seen staining in mouse brain. Any tips? Is anti-MMP2 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
According to literature brain does express MMP2. According to Uniprot.org, MMP2 is expressed in mucosa of stomach, teratocarcinoma testis, brain, melanoma, among other tissues. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-02
Question
I was wanting to use your anti-MMP2 antibody for ELISA for human melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human melanoma identification?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
As indicated on the product datasheet, A00286 anti-MMP2 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-16
Question
We were content with the WB result of your anti-MMP2 antibody. However we have been able to see positive staining in mucosa of stomach isoform 2: cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is MMP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
From what I have seen in literature, mucosa of stomach does express MMP2. Generally MMP2 expresses in isoform 1: secreted, extracellular space,, isoform 2: cytoplasm. mitochondrion. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-10-05
Question
Do you have a BSA free version of anti-MMP2 antibody A00286 available?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP2 antibody A00286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-05
Question
Is a blocking peptide available for product anti-MMP2 antibody (A00286)?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
We do provide the blocking peptide for product anti-MMP2 antibody (A00286). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-19
Question
Is this A00286 anti-MMP2 antibody reactive to the isotypes of MMP2?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
The immunogen of A00286 anti-MMP2 antibody is E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-15
Question
We are interested in to test anti-MMP2 antibody A00286 on human melanoma for research purposes, then I may be interested in using anti-MMP2 antibody A00286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
The products we sell, including anti-MMP2 antibody A00286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-24
Question
Can you help my question with product A00286, anti-MMP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Miller
Verified customer
Asked: 2015-11-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00286 anti-MMP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-11-02
