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Product Info Summary
| SKU: | EK1109 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Rat Cystatin C/CST3 ELISA Kit PicoKine®
View all Cystatin C ELISA kits
SKU/Catalog Number
EK1109
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Cystatin C/CST3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cst3 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Cystatin C/CST3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1109)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M1-A140
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1109 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1109 is reactive to Cst3 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1109 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cystatin C
Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1109-CAP | Anti-Rat Cst3 Pre-coated 96-well strip microplate | 1 |
| EK1109-ST | Rat Cst3 Standard | 2 vials, 20 ng/tube |
| EK1109-DA | Rat Cst3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.009 | 0.285 | 0.525 | 0.816 | 1.222 | 1.368 | 1.587 | 1.951 |
Data Example Images
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Rat Cystatin C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cystatin C, For serum, dilution ratio is 1:500 and concentration is 1122-3213 ng/ml.
For plasma, dilution ratio is 1:500 and concentration is 770-2464 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Cystatin C/CST3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 824 | 2517 | 8104 | 756 | 2556 | 7350 |
| Standard deviation | 34.6 | 128.36 | 445.72 | 39.31 | 146.49 | 470.40 |
| CV (%) | 4.2% | 6.4% | 5.5% | 5.2% | 5.7% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 824 | 793 | 785 | 735 | 784 | 31.94 | 4% |
| Sample 2 | 2517 | 2693 | 2642 | 2687 | 2634 | 70.78 | 2.6% |
| Sample 3 | 8104 | 8014 | 8243 | 8197 | 8139 | 88.07 | 1% |
Protein Target Info & Infographic
Gene/Protein Information For Cst3 (Source: Uniprot.Org, NCBI)
Gene Name
Cst3
Full Name
Cystatin-C
Weight
15437 MW
Superfamily
cystatin family
Alternative Names
Cystatin-C;Cystatin-3;Cst3; CST3 ARMD11, HEL-S-2 cystatin C cystatin-C|bA218C14.4 (cystatin C)|cystatin 3|epididymis secretory protein Li 2|gamma-trace|neuroendocrine basic polypeptide|post-gamma-globulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cst3, check out the Cst3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cst3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Cystatin C/CST3 ELISA Kit PicoKine® (EK1109)
Hello CJ!
EK1109 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Safety and Efficacy of Combined Low-Dose Lithium and Low-Dose Aspirin: A Pharmacological and Behavioral Proof-of-Concept Study in Rats
Epoxyeicosatrienoic Acid-Based Therapy Attenuates the Progression of Postischemic Heart Failure in Normotensive Sprague-Dawley but Not in Hypertensive Ren-2 Transgenic Rats
The effect of tadalafil therapy on kidney damage caused by sepsis in a polymicrobial septic model induced in rats: a biochemical and histopathological study
The effect of trimetazidine on renal oxidative stress in partial and complete ureteral obstructions in a rat model
Benli E, Ayyildiz SN, Cirrik S, Kokt
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Rat Cystatin C/CST3 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Cystatin C using the EK1109 Rat Cystatin C Picokine® ELISA Kit?
T. Johnson
Verified customer
Asked: 2020-11-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-11-09
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Cystatin C in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
H. Clark
Verified customer
Asked: 2020-05-19
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cystatin C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-19
Question
Q: can I use citrate plasma as samples in Rat Cystatin C Picokine® ELISA Kit (Catalog # EK1109)?
Verified Customer
Verified customer
Asked: 2017-11-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Cystatin C causing degradation of its protein structure. Cystatin C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Cystatin C ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-11-19
Question
Q: which procedure should I follow in order to thaw whole blood sample for Cystatin C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cystatin C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-09-28
Question
Q: if the enzyme conjugated Cystatin C antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cystatin C antigen?
T. White
Verified customer
Asked: 2017-05-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-05-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Young
Verified customer
Asked: 2016-02-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-02-10
