Click image to see more details
Product Info Summary
| SKU: | EK0855 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and urine. |
Product info
Product Name
Rat Lipocalin-2/NGAL ELISA Kit PicoKine®
View all Lipocalin-2/NGAL ELISA kits
SKU/Catalog Number
EK0855
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0855)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-N198
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0855 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0855 is reactive to Lcn2 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and urine.
Application Guarantee
EK0855 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lipocalin-2/NGAL
Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0855-CAP | Anti-Rat Lcn2 Pre-coated 96-well strip microplate | 1 |
| EK0855-ST | Rat Lcn2 Standard | 2 vials, 10 ng/tube |
| EK0855-DA | Rat Lcn2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.067 | 0.135 | 0.181 | 0.312 | 0.484 | 0.780 | 1.249 | 1.737 |
Data Example Images
Click image to see more details
Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lipocalin-2/NGAL, Dilution ratio of 1:500, concentration in serum is 3.2-4.8 ng/ml..
Some articles we found to cite concentrations of Lipocalin-2/NGAL in samples: 24551041 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Lipocalin-2/NGAL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 121 | 750 | 3188 | 126 | 727 | 3167 |
| Standard deviation | 6.05 | 50.25 | 168.96 | 7.3 | 56.7 | 177.35 |
| CV (%) | 5% | 6.7% | 5.3% | 5.8% | 7.8% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 121 | 118 | 102 | 117 | 114 | 7.36 | 6.4% |
| Sample 2 | 750 | 751 | 693 | 740 | 733 | 23.77 | 3.2% |
| Sample 3 | 3188 | 2684 | 2847 | 3144 | 2965 | 208.95 | 7% |
Protein Target Info & Infographic
Gene/Protein Information For Lcn2 (Source: Uniprot.Org, NCBI)
Gene Name
Lcn2
Full Name
Neutrophil gelatinase-associated lipocalin
Weight
22476 MW
Superfamily
calycin superfamily
Alternative Names
Neutrophil gelatinase-associated lipocalin;NGAL;Alpha-2-microglobulin-related protein;Alpha-2U globulin-related protein;Lipocalin-2;Siderocalin LCN2;p25;Lcn2; LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Lcn2, check out the Lcn2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lcn2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (EK0855)
Hello CJ!
EK0855 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats
Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis
Proteome Analysis of Urinary Biomarkers in Acute Hypercoagulable State Rat Model
Does remifentanil attenuate renal ischemia–reperfusion injury better than dexmedetomidine in rat kidney?
Can remote ischemic preconditioning counteract the renal functional deterioration attributable to partial nephrectomy under warm ischemia? Results of an animal study
Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning
Astaxanthin protects against early acute kidney injury in severely burned rats by inactivating the TLR4/MyD88/NF-κB axis and upregulating heme oxygenase-1
Guo S,Guo L,Fang Q,Yu M,Zhang L,You C,Wang X,Liu Y,Han C.Astaxanthin protects against early acute kidney injury in severely burned rats by inactivating the TLR4/MyD88/NF-κB axis and upregulating heme oxygenase-1.Sci Rep.2021 Mar 23;11 (1):6679.doi:10.1038/s41598-021-86146-w.PMID:33758309;PMCID:PMC7988001.
Species: Rat
EK0855 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Songxue Guo,Linsen Guo,Quan Fang et al.Astaxanthin Protects Against Early Acute Kidney Injury in Severely-Burned Rats Through Inactivating TLR4/MyD88/NF-kB Axis and Upregulating Heme Oxygenase-1, 07 October 2020, PREPRINT (Version 1) available at Res
Species: Rat
EK0855 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Rat Lipocalin-2/NGAL ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Rat Lipocalin-2/NGAL ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
10 Customer Q&As for Rat Lipocalin-2/NGAL ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-22
Question
Can EK0855 be used for stool supernatant samples?
Verified customer
Asked: 2020-01-17
Answer
Our lab hasn't worked on stool supernatant samples with the Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (EK0855). We suggest to run a pilot test. In theory, the kit is able to detect the target protein if the expression level of NGAL in the stool supernatant samples falls in the assay range of EK0855.
Boster Scientific Support
Answered: 2020-01-17
Question
Q: how do I analyze ELISA data? I measured Lipocalin-2 level in serum.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-01-14
Question
Is there supposed to be a washstep in between step 5 and step 6 of the ELISA protocols in the datasheet?
Verified customer
Asked: 2019-04-09
Answer
There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-07-17
Question
I am preparing to use your Rat Lcn2 ELISA cat#EK0855. In reading the procedure I noticed there is not a wash after step 5 and before step 6. This is after incubating samples/standard and before addition of the 1xbiotinylated Anti-Rat Lcn2 antibody. Is this correct?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.
Boster Scientific Support
Answered: 2017-06-14
Question
Q: how to thaw whole blood sample for Lipocalin-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Lipocalin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-06-14
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-04-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-04-12
Question
The customer would like to know if (#LB001-02 from Welgene) can be used as washing buffer for EK0855 kit. The composition is 200mg/L KCl, 200mg/L KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4
Verified Customer
Verified customer
Asked: 2014-12-26
Answer
The recipe of our wash buffer is different from that of LB002-01. We haven't used the same buffer as LB002-01. And we do not suggest the customer use it. Please use the wash buffer included in the kit. Or prepare wash buffer as the method described below. Washing buffer (neutral PBS or TBS). ? Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450?l of purified acetic acid or 700?l of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. ? Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Boster Scientific Support
Answered: 2014-12-26
Question
Q: is it okay to use citrate plasma as samples in Rat Lipocalin-2 Picokine® ELISA Kit (Catalog # EK0855)?
K. Scott
Verified customer
Asked: 2014-05-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Lipocalin-2 causing disruption of its protein structure. Lipocalin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Lipocalin-2 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-05-18
