Human Cystatin C/CST3 ELISA Kit PicoKine®

Product Name

Human Cystatin C/CST3 ELISA Kit PicoKine®

View all Cystatin C ELISA kits

SKU/Catalog Number

EK0678

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Cystatin C/CST3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Cystatin C/CST3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0678)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: M1-A146

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0678 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0678 is reactive to CST3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EK0678 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cystatin C

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Kit Components

Catalog Number	Description	Quantity
EK0678-CAP	Anti-Human CST3 Pre-coated 96-well strip microplate	1
EK0678-ST	Human CST3 Standard	2 vials, 20 ng/tube
EK0678-DA	Human CST3 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.036	0.095	0.185	0.290	0.581	0.952	1.350	1.923

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cystatin C, Dilution ratio of 1:500, concentration in serum and plasma is around 1500 ng/ml..

Some articles we found to cite concentrations of Cystatin C in samples: 24963810, 25216761, 24821156, 24661756, 22120484 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Cystatin C/CST3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.

Gene/Protein Information For CST3 (Source: Uniprot.Org, NCBI)

Alternative Names

Cystatin-C;Cystatin-3;Gamma-trace;Neuroendocrine basic polypeptide;Post-gamma-globulin;CST3; CST3 ARMD11, HEL-S-2 cystatin C cystatin-C|bA218C14.4 (cystatin C)|cystatin 3|epididymis secretory protein Li 2|gamma-trace|neuroendocrine basic polypeptide|post-gamma-globulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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