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Product Info Summary
| SKU: | EK0679 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma ( heparin, EDTA) and urine. |
Product info
Product Name
Mouse Cystatin C/CST3 ELISA Kit PicoKine®
View all Cystatin C ELISA kits
SKU/Catalog Number
EK0679
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Cystatin C/CST3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cst3 in cell culture supernatants, serum, plasma ( heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Cystatin C/CST3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0679)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M1-A140
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0679 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0679 is reactive to Cst3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma ( heparin, EDTA) and urine.
Application Guarantee
EK0679 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cystatin C
Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0679-CAP | Anti-Mouse Cst3 Pre-coated 96-well strip microplate | 1 |
| EK0679-ST | Mouse Cst3 Standard | 2 vials, 20 ng/tube |
| EK0679-DA | Mouse Cst3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.040 | 0.148 | 0.294 | 0.544 | 0.802 | 1.134 | 1.695 | 1.962 |
Data Example Images
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Mouse Cystatin C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cystatin C, Dilution ratio of 1:200, concentration in serum and plasma is around 800 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Cystatin C/CST3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 437 | 2310 | 9815 | 464 | 2268 | 10497 |
| Standard deviation | 27.96 | 97.02 | 706.68 | 34.8 | 106.59 | 871.25 |
| CV (%) | 6.4% | 4.2% | 7.2% | 7.5% | 4.7% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 437 | 434 | 446 | 503 | 455 | 28.06 | 6.1% |
| Sample 2 | 2310 | 2387 | 2135 | 2376 | 2302 | 100.81 | 4.3% |
| Sample 3 | 9815 | 8364 | 8977 | 9330 | 9121 | 528.93 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For Cst3 (Source: Uniprot.Org, NCBI)
Gene Name
Cst3
Full Name
Cystatin-C
Weight
15531 MW
Superfamily
cystatin family
Alternative Names
Cystatin-C;Cystatin-3;Cst3; CST3 ARMD11, HEL-S-2 cystatin C cystatin-C|bA218C14.4 (cystatin C)|cystatin 3|epididymis secretory protein Li 2|gamma-trace|neuroendocrine basic polypeptide|post-gamma-globulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cst3, check out the Cst3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cst3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Cystatin C/CST3 ELISA Kit PicoKine® (EK0679)
Hello CJ!
EK0679 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Activated iRhom2 drives prolonged PM2.5 exposure-triggered renal injury in Nrf2-defective mice
Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
Species: Rat
EK0679 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Activated iRhom2 drives prolonged PM2.5 exposure-triggered renal injury in Nrf2-defective mice
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse Cystatin C/CST3 ELISA Kit PicoKine®
Question
Hi, do you have any data for the recommended dilution of mouse urine for kit EK0679?
Verified customer
Asked: 2022-10-30
Answer
The recommended dilution ratio of mouse urine for kit EK0679 is 1:50.
Boster Scientific Support
Answered: 2022-10-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Cystatin C using the EK0679 Mouse Cystatin C Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-10-29
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Cystatin C in Mouse urine? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-10-22
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cystatin C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-10-22
Question
Q: What is the optimal O.D. value for Cystatin C ELISA kit? I performed your Cystatin C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Cystatin C even though the O.D. values are not very high?
M. Turner
Verified customer
Asked: 2020-02-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cystatin C ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cystatin C that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-01
Question
Q: if the enzyme conjugated Cystatin C antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cystatin C antigen?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-06-26
Question
Q: Can Cystatin C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-07-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-06-29
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Cystatin C level in urine.
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-22
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
A. Allen
Verified customer
Asked: 2014-05-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-05-08
