Click image to see more details
Product Info Summary
| SKU: | EK0853 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) , saliva and urine. |
Product info
Product Name
Human Lipocalin-2/NGAL ELISA Kit PicoKine®
View all Lipocalin-2/NGAL ELISA kits
SKU/Catalog Number
EK0853
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Lipocalin-2/NGAL ELISA Kit PicoKine® (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Lipocalin-2/NGAL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0853)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-G198
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0853 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0853 is reactive to LCN2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) , saliva and urine.
Application Guarantee
EK0853 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lipocalin-2/NGAL
Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0853-CAP | Anti-Human LCN2 Pre-coated 96-well strip microplate | 1 |
| EK0853-ST | Human LCN2 Standard | 2 vials, 10 ng/tube |
| EK0853-DA | Human LCN2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.078 | 0.202 | 0.410 | 0.547 | 0.831 | 0.953 | 1.409 | 1.885 |
Data Example Images
Click image to see more details
Human Lipocalin-2/NGAL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lipocalin-2/NGAL, Dilution ratio of 1:100, concentration in serum and plasma is 40-100 ng/ml..
Some articles we found to cite concentrations of Lipocalin-2/NGAL in samples: 24528623 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Lipocalin-2/NGAL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 256 | 1510 | 5422 | 254 | 1537 | 5138 |
| Standard deviation | 19.2 | 81.54 | 281.94 | 19.05 | 84.53 | 323.69 |
| CV (%) | 7.5% | 5.4% | 5.2% | 7.5% | 5.5% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 256 | 230 | 223 | 260 | 242 | 16 | 6.6% |
| Sample 2 | 1510 | 1658 | 1546 | 1807 | 1630 | 115.72 | 7% |
| Sample 3 | 5422 | 5479 | 5362 | 4730 | 5248 | 302.05 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For LCN2 (Source: Uniprot.Org, NCBI)
Gene Name
LCN2
Full Name
Neutrophil gelatinase-associated lipocalin
Weight
22588 MW
Superfamily
calycin superfamily
Alternative Names
Neutrophil gelatinase-associated lipocalin;NGAL;25 kDa alpha-2-microglobulin-related subunit of MMP-9;Lipocalin-2;Oncogene 24p3;Siderocalin LCN2;p25;LCN2;HNL, NGAL; LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LCN2, check out the LCN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Lipocalin-2/NGAL ELISA Kit PicoKine® (EK0853)
Hello CJ!
EK0853 has been cited in 27 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Urinary kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin as early biomarkers for predicting vancomycin-associated acute kidney injury: a prospective study
Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients
Can serum NGAL levels be used as an inflammation marker on hemodialysis patients with permanent catheter?
Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Schönlein Purpura Patients with Renal Involvement
The value of plasma pro-enkephalin and adrenomedullin for the prediction of sepsis-associated acute kidney injury in critically ill patients
Predictive role of urinary neutrophil gelatinase-associated lipocalin in lupus nephritis:
Association of Serum Lipocalin-2 Concentrations with Psoriasis and Psoriatic Arthritis: An Updated Meta-Analysis
Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis
Prognostic impact of established and novel renal function biomarkers in myocardial infarction with cardiogenic shock: A biomarker substudy of the IABP-SHOCK II-trial
Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human Lipocalin-2/NGAL ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human Lipocalin-2/NGAL ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Human Lipocalin-2/NGAL ELISA Kit PicoKine®
Question
Could you please let me know the animal derive components/buffers and the species these are derived from for your Kit Human Lipocalin-2 ELISA Kit (NGAL) EK0853? Many thanks in advance for your time and help.
Verified customer
Asked: 2022-08-17
Answer
Thanks for your questions. Species: Goat (capture and detection antibodies) Bovine(Standard, Sample Diluent, Antibody Diluent and Avidin-Biotin-Peroxidase Diluent contain BSA)
Boster Scientific Support
Answered: 2022-08-17
Question
Does EK0853 contain two antibodies from goat as indicated in your protocol booklet?
Verified customer
Asked: 2022-08-09
Answer
The Human Lipocalin-2/NGAL ELISA Kit PicoKine® (EK0853) contains two antibodies from goat.
Boster Scientific Support
Answered: 2022-08-10
Question
What is the animal derive components/buffers and the species these are derived from in EK0853?
Verified customer
Asked: 2022-07-27
Answer
For the Human Lipocalin-2/NGAL ELISA Kit PicoKine® (EK0853), the species for the capture and detection antibodies is Goat. The Standard, Sample Diluent, Antibody Diluent and Avidin-Biotin-Peroxidase Diluent contain BSA is Bovine.
Boster Scientific Support
Answered: 2022-07-28
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Lipocalin-2 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-05-21
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Lipocalin-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-21
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
V. Jackson
Verified customer
Asked: 2019-10-23
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-10-23
Question
Q: What is the optimal O.D. value for Lipocalin-2 ELISA kit? I used your Lipocalin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Lipocalin-2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lipocalin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lipocalin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-07-22
Question
Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?
S. Roberts
Verified customer
Asked: 2019-05-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-23
Question
Q: how to analyze ELISA data? I have performed Lipocalin-2 level in serum.
Verified Customer
Verified customer
Asked: 2019-05-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-18
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-03-06
Question
Q: can I use citrate plasma as samples in Human Lipocalin-2 Picokine® ELISA Kit (Catalog # EK0853)?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Lipocalin-2 causing disruption of its protein structure. Lipocalin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Lipocalin-2 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-21
Question
Q: which procedure should I follow in order to thaw whole blood sample for Lipocalin-2 ELISA after freezing?
Z. Jones
Verified customer
Asked: 2018-04-19
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Lipocalin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-04-19
