Product Info Summary
SKU: | PB9608 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Lipocalin 2/LCN2 Antibody Picoband®
View all Lipocalin-2/NGAL Antibodies
SKU/Catalog Number
PB9608
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lipocalin 2/LCN2 Antibody Picoband® catalog # PB9608. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipocalin 2/LCN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9608)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200). Mouse Lipocalin 2 shares 62 % and 81.1 % amino acid (aa) sequence identity with human and rat Lipocalin 2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9608 is reactive to Lcn2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
22875 MW
Background of Lipocalin-2/NGAL
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9608 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Western blot, 0.1-0.5μg/ml
Positive Control
WB: mouse spleen tissue
IHC: Mouse Spleen tissue, Rat Spleen tissue
IHC-F: mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9608) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
Click image to see more details
Figure 2. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in frozen section of mouse lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in frozen section of rat lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).
Lipocalin 2 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Lcn2 (Source: Uniprot.org, NCBI)
Gene Name
Lcn2
Full Name
Neutrophil gelatinase-associated lipocalin
Weight
22875 MW
Superfamily
calycin superfamily
Alternative Names
Neutrophil gelatinase-associated lipocalin;NGAL;Lipocalin-2;SV-40-induced 24P3 protein;Siderocalin LCN2;p25;Lcn2; LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lcn2, check out the Lcn2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lcn2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lipocalin 2/LCN2 Antibody Picoband® (PB9608)
Hello CJ!
PB9608 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of neutrophil gelatinase-associated lipocalin in low osmolar contrast-induced nephropathy in rats and the effect of N-acetylcysteine
Late intervention with the small molecule BB3 mitigates postischemic kidney injury
Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...
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