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Product Info Summary
| SKU: | EK0767 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human GDF-15 ELISA Kit PicoKine®
SKU/Catalog Number
EK0767
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GDF-15 ELISA Kit PicoKine® (96 Tests). Quantitate Human GDF15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GDF-15 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0767)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A197-I308
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0767 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0767 is reactive to GDF15 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0767 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDF-15
GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0767-CAP | Anti-Human GDF15 Pre-coated 96-well strip microplate | 1 |
| EK0767-ST | Human GDF15 Standard | 2 vials, 10 ng/tube |
| EK0767-DA | Human GDF15 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.002 | 0.071 | 0.136 | 0.309 | 0.480 | 0.849 | 1.352 | 1.922 |
Data Example Images
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Human GDF-15 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GDF-15, Dilution ratio of 1:1, concentration in serum and plasma is 269-1272 pg/ml..
Some articles we found to cite concentrations of GDF-15 in samples: 24748192 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GDF-15 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 53 | 252 | 1068 | 53 | 266 | 1115 |
| Standard deviation | 3.55 | 16.12 | 74.76 | 3.92 | 20.21 | 81.39 |
| CV (%) | 6.7% | 6.4% | 7% | 7.4% | 7.6% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDF-15 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 53 | 56 | 49 | 57 | 53 | 3.11 | 5.8% |
| Sample 2 | 252 | 245 | 249 | 271 | 254 | 9.98 | 3.9% |
| Sample 3 | 1068 | 1033 | 966 | 984 | 1012 | 40.23 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For GDF15 (Source: Uniprot.Org, NCBI)
Gene Name
GDF15
Full Name
Growth/differentiation factor 15
Weight
34140 MW
Superfamily
TGF-beta family
Alternative Names
Growth/differentiation factor 15;GDF-15;Macrophage inhibitory cytokine 1;MIC-1;NSAID-activated gene 1 protein;NAG-1;NSAID-regulated gene 1 protein;NRG-1;Placental TGF-beta;Placental bone morphogenetic protein;Prostate differentiation factor;GDF15;MIC1, PDF, PLAB, PTGFB; GDF15 GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB growth differentiation factor 15 growth/differentiation factor 15|NRG-1|NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|NSAID-activated gene 1 protein|NSAID-regulated gene 1 protein|PTGF-beta|macrophage inhibitory cytokine 1|non-steroidal anti-inflammatory drug-activated gene-1|placental TGF-beta|placental bone morphogenetic protein|prostate differentiation factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDF15, check out the GDF15 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDF15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GDF-15 ELISA Kit PicoKine® (EK0767)
Hello CJ!
EK0767 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Maternal levels of growth differentiation factor-15 in patients with preeclampsia
Maternal levels of growth differentiation factor-15 in patients with preeclampsia
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human GDF-15 ELISA Kit PicoKine®
Question
Does the Human GDF-15 ELISA Kit PicoKine® detect a specific GDF-15 variant (such as HD andDD)?
Verified customer
Asked: 2022-09-08
Answer
The immunogen sequence for antibody pair in EK0767 is A197-I308. Our lab hasn't design the kit for any specific GDF-15 variant. The kit might cross-react with some variant.
Boster Scientific Support
Answered: 2022-09-09
Question
How is serum or plasma samples be diluted for EK0767?
Verified customer
Asked: 2022-06-22
Answer
For the Human GDF-15 ELISA Kit PicoKine® (EK0767), sample dilution ratios should be determined by a pilot study (run a serial dilution of samples and see which dilution ratio results in the idea O.D., near the middle of the standard range). In general, high concentration samples can be dilutioned by 1:100, mid concentration samples 1:10, low concentration samples 1:2 or neat.
Boster Scientific Support
Answered: 2022-06-22
Question
Can EK0767 preferentially detect one H6D variant of GDF15 over the other?
Verified customer
Asked: 2021-02-12
Answer
Our lab hasn't experimentally validated if the Human GDF-15 ELISA Kit PicoKine™ (EK0767) preferentially detects one H6D variant of GDF15 over the other. The immunogen sequence of the antibody pairs in the kit is A197-I308 (Uniprot ID: Q99988).
Boster Scientific Support
Answered: 2021-02-19
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: is it okay to use citrate plasma as samples in Human GDF-15 Picokine® ELISA Kit (Catalog # EK0767)?
T. Mehta
Verified customer
Asked: 2019-09-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of GDF-15 causing degradation of its protein structure. GDF-15 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the GDF-15 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-15
Question
Q: What is the optimal O.D. value for GDF-15 ELISA kit? I performed your GDF-15 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GDF-15 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GDF-15 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GDF-15 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-09-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement GDF-15 using the EK0767 Human GDF-15 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-10
Question
A customer wanted to know the concentration of the detection antibody from the Human GDF-15 kit? The concentration of the current lot of EK0767 would be fine.
Verified Customer
Verified customer
Asked: 2016-12-05
Answer
The concentration of detection antibody in the kit is 0.5ug/ml.
Boster Scientific Support
Answered: 2016-12-05
Question
Could you provide the suggested working dilution ratios for our validated sample types: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA) for Human GDF-15 #EK0767 ?
Verified Customer
Verified customer
Asked: 2014-09-01
Answer
Please see the following suggested working dilution ratios for the samples. serum and plasma (heparin, EDTA) 1:1 cell culture supernates, cell lysates 1:20
Boster Scientific Support
Answered: 2014-09-01
