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Product Info Summary
| SKU: | EK0502 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, citrate). |
Product info
Product Name
Mouse sE-Selectin ELISA Kit PicoKine®
View all E-Selectin/CD62E ELISA kits
SKU/Catalog Number
EK0502
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse sE-Selectin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sele in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse sE-Selectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0502)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: W22-P557
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0502 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0502 is reactive to Sele in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, citrate).
Application Guarantee
EK0502 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of E-Selectin/CD62E
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0502-CAP | Anti-Mouse Sele Pre-coated 96-well strip microplate | 1 |
| EK0502-ST | Mouse Sele Standard | 2 vials, 10 ng/tube |
| EK0502-DA | Mouse Sele Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.013 | 0.041 | 0.080 | 0.201 | 0.368 | 0.751 | 1.474 | 2.056 |
Data Example Images
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Mouse sE-Selectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect E-Selectin/CD62E, Dilution ratio of 1:10, concentration in serum and plasma is 5.2-9.0 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse sE-Selectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1580 | 4329 | 277 | 1451 | 4721 |
| Standard deviation | 11.96 | 105.86 | 186.14 | 16.62 | 108.82 | 250.21 |
| CV (%) | 4.4% | 6.7% | 4.3% | 6% | 7.5% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of E-Selectin/CD62E in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 272 | 280 | 290 | 284 | 281 | 6.53 | 2.3% |
| Sample 2 | 1580 | 1395 | 1401 | 1536 | 1478 | 81.52 | 5.5% |
| Sample 3 | 4329 | 4374 | 4283 | 4168 | 4288 | 76.65 | 1.7% |
Protein Target Info & Infographic
Gene/Protein Information For Sele (Source: Uniprot.Org, NCBI)
Gene Name
Sele
Full Name
E-selectin
Weight
66750 MW
Superfamily
selectin/LECAM family
Alternative Names
E-selectin;CD62 antigen-like family member E;Endothelial leukocyte adhesion molecule 1;ELAM-1;Leukocyte-endothelial cell adhesion molecule 2;LECAM2;CD62E;Sele;Elam-1; SELE CD62E, ELAM, ELAM1, ESEL, LECAM2 selectin E E-selectin|CD62 -like family member E|ELAM-1|endothelial adhesion molecule 1|endothelial leukocyte adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Sele, check out the Sele Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sele: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse sE-Selectin ELISA Kit PicoKine® (EK0502)
Hello CJ!
EK0502 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of Orai1-mediated Ca2+ entry limits endothelial cell inflammation by suppressing calcineurin-NFATc4 signaling pathway
Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf...
Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...
Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan.
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Mouse sE-Selectin ELISA Kit PicoKine®
Question
Is there any salt in EK0502?
Verified customer
Asked: 2022-05-31
Answer
There is salt (PBS) in the Mouse SE-Selectin ELISA Kit PicoKine® (EK0502).
Boster Scientific Support
Answered: 2022-06-01
Question
What is the concentration of sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) if it is contained in EK0502?
Verified customer
Asked: 2022-05-27
Answer
The Mouse SE-Selectin ELISA Kit PicoKine® (EK0502) doesn't contain any sodium azide or thimerosal and 2-mercaptoethanol. It contains Proclin 300. Please see the MSDS for detailed concentration for each component. https://www.bosterbio.com/msds?sku=EK0502
Boster Scientific Support
Answered: 2022-05-29
Question
Is the protein in EK0502 derived from the Baculovirus expression system?
Verified customer
Asked: 2022-05-27
Answer
The protein in the Mouse SE-Selectin ELISA Kit PicoKine® (EK0502) is not derived from the Baculovirus expression system.
Boster Scientific Support
Answered: 2022-05-29
Question
What are the components of the reaction stop solution of EK0502?
Verified customer
Asked: 2022-05-27
Answer
The stop solution in the Mouse SE-Selectin ELISA Kit PicoKine® (EK0502) contains 2% Sulphuric acid.
Boster Scientific Support
Answered: 2022-05-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD62E using the EK0502 Mouse CD62E Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-17
Question
Q: if the enzyme conjugated CD62E antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD62E antigen?
H. Martin
Verified customer
Asked: 2020-04-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-02
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD62E in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD62E ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-25
Question
Q: Can CD62E ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-02-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Harris
Verified customer
Asked: 2018-01-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD62E ELISA after freezing?
M. Mangal
Verified customer
Asked: 2017-07-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD62E for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-07-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Jackson
Verified customer
Asked: 2016-12-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-12-12
Question
Q: is it okay to use EDTA plasma as samples in Mouse CD62E Picokine® ELISA Kit (Catalog # EK0502)?
Verified Customer
Verified customer
Asked: 2016-09-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD62E causing disruption of its protein structure. CD62E may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD62E ELISA, treating samples with different anticoagulants and decided that heparin or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-07-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-07-08
