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Product Info Summary
| SKU: | EK0974 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®
View all Chitinase 3-like 1 ELISA kits
SKU/Catalog Number
EK0974
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHI3L1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0974)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y22-T383
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0974 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0974 is reactive to CHI3L1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0974 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chitinase 3-like 1
Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0974-CAP | Anti-Human CHI3L1 Pre-coated 96-well strip microplate | 1 |
| EK0974-ST | Human CHI3L1 Standard | 2 vials, 10 ng/tube |
| EK0974-DA | Human CHI3L1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.002 | 0.065 | 0.127 | 0.234 | 0.435 | 0.895 | 1.407 | 1.819 |
Data Example Images
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Human Chitinase 3-like 1/YKL-40 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Chitinase 3-like 1, Dilution ratio of 1:50, concentration in serum and plasma is 12.72-112.2 ng/ml..
Some articles we found to cite concentrations of Chitinase 3-like 1 in samples: 25597749, 25740558, 25253160, 21915293 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 90 | 671 | 1314 | 87 | 650 | 1207 |
| Standard deviation | 4.95 | 27.51 | 69.64 | 6.17 | 38.35 | 80.86 |
| CV (%) | 5.5% | 4.1% | 5.3% | 7.1% | 5.9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chitinase 3-like 1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 84 | 86 | 89 | 87 | 2.38 | 2.7% |
| Sample 2 | 671 | 639 | 681 | 646 | 659 | 17.29 | 2.6% |
| Sample 3 | 1314 | 1141 | 1343 | 1237 | 1258 | 78.24 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For CHI3L1 (Source: Uniprot.Org, NCBI)
Gene Name
CHI3L1
Full Name
Chitinase-3-like protein 1
Weight
42625 MW
Superfamily
glycosyl hydrolase 18 family
Alternative Names
Chitinase-3-like protein 1;39 kDa synovial protein;Cartilage glycoprotein 39;CGP-39;GP-39;hCGP-39;YKL-40;CHI3L1; CHI3L1 ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39 chitinase 3 like 1 chitinase-3-like protein 1|39 kDa synovial protein|cartilage glycoprotein 39|chitinase 3-like 1 (cartilage glycoprotein-39)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHI3L1, check out the CHI3L1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHI3L1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (EK0974)
Hello CJ!
EK0974 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum YKL-40 predicts long-term outcome in patients undergoing primary percutaneous coronary intervention for ST-segment elevation myocardial infarction
Comparison between galectin-3 and YKL-40 levels for the assessment of liver fibrosis in cirrhotic patients
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
A. Banerjee
Verified customer
Asked: 2020-05-11
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-11
Question
Q: which procedure should I follow in order to thaw whole blood sample for Chitinase 3-like 1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Chitinase 3-like 1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-04-21
Question
What is the exact sensitivity of EK0974?
Verified customer
Asked: 2019-09-18
Answer
The sensitivity of the Human Chitinase 3-Like 1/YKL-40 ELISA Kit PicoKine™ (EK0974) is <10pg/ml.
Boster Scientific Support
Answered: 2019-09-18
Question
Q: how to analyze ELISA data? I have obtained Chitinase 3-like 1 level in plasma.
A. Taylor
Verified customer
Asked: 2019-05-17
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-17
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
L. Parker
Verified customer
Asked: 2018-10-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-21
Question
Q: Can Chitinase 3-like 1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Maharaj
Verified customer
Asked: 2018-08-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-29
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Chitinase 3-like 1 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Chitinase 3-like 1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-25
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Chitinase 3-like 1 using the EK0974 Human Chitinase 3-like 1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-02-02
Question
Q: can I use citrate plasma as samples in Human Chitinase 3-like 1 Picokine® ELISA Kit (Catalog # EK0974)?
J. Thompson
Verified customer
Asked: 2017-09-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Chitinase 3-like 1 causing degradation of its protein structure. Chitinase 3-like 1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Chitinase 3-like 1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-09-29
