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Product Info Summary
| SKU: | PA2146 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Macrophage metalloelastase MMP12 Antibody Picoband®
SKU/Catalog Number
PA2146
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Macrophage metalloelastase MMP12 Antibody catalog # PA2146. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrophage metalloelastase MMP12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2146)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2146 is reactive to MMP12 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
54002 MW
Background of MMP-12
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is though that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2146 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Cell, HEPA Cell, HELA Cell, K562 Cell, MCF-7 Cell,
Validation Images & Assay Conditions
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Anti-MMP12 antibody, PA2146, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: K562 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For MMP12 (Source: Uniprot.org, NCBI)
Gene Name
MMP12
Full Name
Macrophage metalloelastase
Weight
54002 MW
Superfamily
peptidase M10A family
Alternative Names
Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Macrophage metalloelastase MMP12 Antibody Picoband® (PA2146)
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No publications found for PA2146
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Macrophage metalloelastase MMP12 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-MMP12 antibody PA2146. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-06
Question
Do you have a BSA free version of anti-MMP12 antibody PA2146 available?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MMP12 antibody PA2146 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-24
Question
We have seen staining in human amniotic fluid. Any tips? Is anti-MMP12 antibody supposed to stain amniotic fluid positively?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
From literature amniotic fluid does express MMP12. From Uniprot.org, MMP12 is expressed in amniotic fluid, alveolar macrophage, esophagus, among other tissues. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-16
Question
Does PA2146 anti-MMP12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
As indicated on the product datasheet, PA2146 anti-MMP12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-31
Question
I was wanting to use using your anti-MMP12 antibody for negative regulation of type i interferon-mediated signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thanks for your inquiry. This PA2146 anti-MMP12 antibody is tested on smmc cell lysate, hepa cell lysate, hela cell lysate, k562 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-MMP12 antibody PA2146 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-MMP12 antibody for WB for human amniotic fluid on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amniotic fluid identification?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
As indicated on the product datasheet, PA2146 anti-MMP12 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human amniotic fluid in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
See attached the WB image, lot number and protocol we used for amniotic fluid using anti-MMP12 antibody PA2146. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-01
Question
Is this PA2146 anti-MMP12 antibody reactive to the isotypes of MMP12?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The immunogen of PA2146 anti-MMP12 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP12(428-444aa KIDAVFYSKNKYYYFFQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-28
Question
Is a blocking peptide available for product anti-MMP12 antibody (PA2146)?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We do provide the blocking peptide for product anti-MMP12 antibody (PA2146). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-28
Question
My boss were satisfied with the WB result of your anti-MMP12 antibody. However we have seen positive staining in amniotic fluid secreted using this antibody. Is that expected? Could you tell me where is MMP12 supposed to be expressed?
J. Thomas
Verified customer
Asked: 2018-07-31
Answer
Based on literature, amniotic fluid does express MMP12. Generally MMP12 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-07-31
Question
I am interested in to test anti-MMP12 antibody PA2146 on human amniotic fluid for research purposes, then I may be interested in using anti-MMP12 antibody PA2146 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The products we sell, including anti-MMP12 antibody PA2146, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-11
Question
I have a question about product PA2146, anti-MMP12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2146 anti-MMP12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-20
Question
We are currently using anti-MMP12 antibody PA2146 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
J. Jha
Verified customer
Asked: 2016-08-09
Answer
The anti-MMP12 antibody (PA2146) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-09
Question
Will anti-MMP12 antibody PA2146 work for WB with amniotic fluid?
Z. Dhar
Verified customer
Asked: 2014-01-23
Answer
According to the expression profile of amniotic fluid, MMP12 is highly expressed in amniotic fluid. So, it is likely that anti-MMP12 antibody PA2146 will work for WB with amniotic fluid.
Boster Scientific Support
Answered: 2014-01-23
