Monkey primate Epiregulin ELISA Kit PicoKine®

Product Name

Monkey primate Epiregulin ELISA Kit PicoKine®

View all Epiregulin ELISA kits

SKU/Catalog Number

EK1394-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate Epiregulin ELISA Kit PicoKine® (96 Tests). Quantitate Monkey EREG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate Epiregulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1394-PR)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: V60-L108

Sensitivity

<10 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Standard Dilution Instructions

See datasheet of EK1394-PR for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1394-PR is reactive to EREG in Monkey samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1394-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Epiregulin

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Kit Components

Catalog Number	Description	Quantity
EK1394-PR-CAP	Anti-Monkey EREG Pre-coated 96-well strip microplate	1
EK1394-PR-ST	Monkey EREG Standard	2 vials, 10 ng/tube
EK1394-PR-DA	Monkey EREG Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.063	0.103	0.180	0.301	0.531	0.8779	1.426	2.062

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate Epiregulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For EREG (Source: Uniprot.Org, NCBI)

Alternative Names

Proepiregulin;Epiregulin;EPR;EREG; EREG EPR, ER, Ep epiregulin proepiregulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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