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Product Info Summary
| SKU: | EK1394 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Epiregulin ELISA Kit PicoKine®
View all Epiregulin ELISA kits
SKU/Catalog Number
EK1394
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Epiregulin ELISA Kit PicoKine® (96 Tests). Quantitate Human EREG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Epiregulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1394)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V60-L108
Sensitivity
<2 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
See datasheet of EK1394 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1394 is reactive to EREG in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1394 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Epiregulin
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1394-CAP | Anti-Human EREG Pre-coated 96-well strip microplate | 1 |
| EK1394-ST | Human EREG Standard | 2 vials, 10 ng/tube |
| EK1394-DA | Human EREG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.135 | 0.488 | 0.680 | 0.956 | 1.163 | 1.361 | 1.470 | 1.808 |
Data Example Images
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Human Epiregulin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Epiregulin, Dilution ratio of 1:1, concentration in serum and plasma is 0-12 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Epiregulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 13 | 57 | 113 | 12 | 56 | 105 |
| Standard deviation | 0.78 | 4.21 | 6.19 | 0.94 | 4.48 | 6.4 |
| CV (%) | 6% | 7.4% | 4.6% | 7.9% | 8% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Epiregulin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 13 | 14 | 12 | 12 | 12 | 0.82 | 6.8% |
| Sample 2 | 57 | 55 | 60 | 52 | 56 | 2.91 | 5.1% |
| Sample 3 | 113 | 120 | 129 | 118 | 120 | 5.78 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For EREG (Source: Uniprot.Org, NCBI)
Gene Name
EREG
Full Name
Proepiregulin
Weight
19044 MW
Alternative Names
Proepiregulin;Epiregulin;EPR;EREG; EREG EPR, ER, Ep epiregulin proepiregulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EREG, check out the EREG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EREG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Epiregulin ELISA Kit PicoKine® (EK1394)
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Customer Q&As
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8 Customer Q&As for Human Epiregulin ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human Epiregulin Picokine® ELISA Kit (Catalog # EK1394)?
D. Collins
Verified customer
Asked: 2020-06-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Epiregulin causing degradation of its protein structure. Epiregulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Epiregulin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-13
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Epiregulin level in plasma.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-01-21
Question
Q: how can I thaw whole blood sample for Epiregulin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-10-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Epiregulin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-10-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
N. Liu
Verified customer
Asked: 2018-11-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Epiregulin in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
G. Robinson
Verified customer
Asked: 2018-10-04
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Epiregulin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Patel
Verified customer
Asked: 2017-10-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-10-30
Question
Q: if the enzyme conjugated Epiregulin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Epiregulin antigen?
Verified Customer
Verified customer
Asked: 2016-04-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-04-12
Question
Q: Can Epiregulin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Miller
Verified customer
Asked: 2015-02-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2015-02-17
