Anti-Thymidylate Synthase TYMS Antibody

Product Name

Anti-Thymidylate Synthase TYMS Antibody

View all Thymidylate Synthase Antibodies

SKU/Catalog Number

A04320

Size

2mL

Form

Lyophilized

Description

Boster Bio Anti-Thymidylate Synthase TYMS Antibody (Catalog # A04320). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Thymidylate Synthase TYMS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04320)

Host

Sheep

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04320 is reactive to TYMS in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Observed Molecular Weight

19 kDa

Calculated molecular weight

35716 MW

Background of Thymidylate Synthase

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04320 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:100
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)

Alternative Names

Thymidylate Synthase, HsT422, MGC88736, TMS, TS, TSase, TYMS, TYMS protein TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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