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Product Info Summary
| SKU: | A06692-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-EREG Antibody (C-term)
View all Epiregulin Antibodies
SKU/Catalog Number
A06692-1
Size
80 µl
Description
Boster Bio Anti-EREG Antibody (C-term) (Catalog # A06692-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EREG Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06692-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06692-1 is reactive to EREG in Human
Reconstitution
Calculated molecular weight
19044 Da
Background of Epiregulin
EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06692-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-EREG Antibody (C-term) at 1:1000 dilution
Lane 1: A549 whole cell lysates
Lane 2: human placenta lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 19 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-EREG Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 19 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of lysates from A549, HepG2, U-87 MG cell line (from left to right), using EREG Antibody (C-term). A06692-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Anti-EREG Antibody (C-term)at 1:2000 dilution + A549 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 19 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Western blot analysis of EREG Antibody (C-term) in HepG2 cell line lysates (35ug/lane). EREG (arrow) was detected using the purified Pab.
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A06692-1 staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For EREG (Source: Uniprot.org, NCBI)
Gene Name
EREG
Full Name
Proepiregulin
Weight
19044 Da
Alternative Names
Proepiregulin, Epiregulin, EPR, EREG EREG EPR, ER, Ep epiregulin proepiregulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EREG, check out the EREG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EREG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EREG Antibody (C-term) (A06692-1)
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