Product Info Summary
SKU: | A04732 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MACC1 Antibody
SKU/Catalog Number
A04732
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MACC1 Antibody (Catalog # A04732). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MACC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MACC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04732)
Host
Rabbit
Contents
MACC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1. The immunogen is located within amino acids 430 - 480 of MACC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of MACC1 are known to exist.
Reactive Species
A04732 is reactive to MACC1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
96639 MW
Background of MACC1
Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04732 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MACC1 in mouse liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of MACC1 in human liver tissue with MACC1 antibody at 2.5 μg/mL.
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Immunofluorescence of MACC1 in Human Liver cells with MACC1 antibody at 20 μg/mL.
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Western blot analysis of MACC1 expression in human liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For MACC1 (Source: Uniprot.org, NCBI)
Gene Name
MACC1
Full Name
Metastasis-associated in colon cancer protein 1
Weight
96639 MW
Alternative Names
7A5, SH3BP4L, Metastasis-associated in colon cancer protein 1, SH3 domain-containing protein 7a5 MACC1 7A5, SH3BP4L MET transcriptional regulator MACC1 metastasis-associated in colon cancer protein 1|MACC1, MET transcriptional regulator|SH3 domain-containing protein 7a5|metastasis associated in colon cancer 1|putative binding protein 7a5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MACC1, check out the MACC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MACC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MACC1 Antibody (A04732)
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