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Product Info Summary
| SKU: | EK1394-PO |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Pig |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Pig porcine Epiregulin ELISA Kit PicoKine®
View all Epiregulin ELISA kits
SKU/Catalog Number
EK1394-PO
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Pig porcine Epiregulin ELISA Kit PicoKine® (96 Tests). Quantitate Pig EREG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Pig porcine Epiregulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1394-PO)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V60-L108
Sensitivity
<10 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
See datasheet of EK1394-PO for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1394-PO is reactive to EREG in Pig samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1394-PO is guaranteed for ELISA in Pig by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Epiregulin
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1394-PO-CAP | Anti-Pig EREG Pre-coated 96-well strip microplate | 1 |
| EK1394-PO-ST | Pig EREG Standard | 2 vials, 10 ng/tube |
| EK1394-PO-DA | Pig EREG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.063 | 0.103 | 0.180 | 0.301 | 0.531 | 0.8779 | 1.426 | 2.062 |
Data Example Images
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Pig Epiregulin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Pig porcine Epiregulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 26.2 | 88.7 | 144 | 25.4 | 91.5 | 151 |
| Standard deviation | 1.18 | 7.94 | 6.81 | 1.73 | 7.05 | 11.93 |
| CV (%) | 4.5 | 5.8 | 5.7 | 6.8 | 7.7 | 7.9 |
Protein Target Info & Infographic
Gene/Protein Information For EREG (Source: Uniprot.Org, NCBI)
Gene Name
EREG
Full Name
Proepiregulin
Weight
19044 MW
Alternative Names
Proepiregulin;Epiregulin;EPR;EREG; EREG EPR, ER, Ep epiregulin proepiregulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EREG, check out the EREG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EREG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Pig porcine Epiregulin ELISA Kit PicoKine® (EK1394-PO)
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Customer Q&As
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8 Customer Q&As for Pig porcine Epiregulin ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Epiregulin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Epiregulin antigen?
C. Scott
Verified customer
Asked: 2020-05-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: how to analyze ELISA data? I measured Epiregulin level in serum.
Y. Dhawan
Verified customer
Asked: 2019-12-15
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-12-15
Question
Q: how to thaw whole blood sample for Epiregulin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Epiregulin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-05-08
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-02
Question
Q: What is the optimal O.D. value for Epiregulin ELISA kit? I used your Epiregulin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Epiregulin even though the O.D. values are not very high?
B. Jackson
Verified customer
Asked: 2017-09-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Epiregulin ELISA kit to have sensitivity of 0.5pg/ml, that means the minimum amount of Epiregulin that can be declared/interpreted as positive by the above standard is 0.5pg/ml.
Boster Scientific Support
Answered: 2017-09-20
Question
Q: is it okay to use citrate plasma as samples in Pig Epiregulin Picokine® ELISA Kit (Catalog # EK1394-PO)?
Verified Customer
Verified customer
Asked: 2017-06-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Epiregulin causing disruption of its protein structure. Epiregulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Epiregulin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-06-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-03-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-03-08
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-03-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-03-06
