Proepiregulin Antibodies

Proepiregulin (EREG)

Ligand of the EGF receptor/EGFR and ERBB4.

Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975).

Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357). .

Gene Information

Human
Gene Name:	EREG
Uniprot:	O14944
Entrez:	2069

Family

Belongs to:
No superfamily

Alternative Names

Epiregulin; ER; EREG; proepiregulin

Molecular Weight

Mass (kDA):
19.044 kDA

Genomic Context

Human
Location:	4q13.3
Sequence:	4; NC_000004.12 (74365145..74388749)

Subcellular Localizations

[Epiregulin]: Secreted, extracellular space.; [Proepiregulin]: Cell membrane; Single-pass type I membrane protein.

Diseases

• Neoplasms
• Malignant Neoplasms
• Colorectal Cancer
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Colorectal Neoplasms
• Mammary Neoplasms
• Colorectal Cancer Metastatic
• Carcinoma
• Hyperplasia
• Neoplasm Invasiveness
• Cell Transformation, Neoplastic

• Inflammation
• Liver Neoplasms
• Carcinogenesis
• Lung Neoplasms
• Metastatic Malignant Neoplasm To The Liver
• Hepatitis B
• Infertility

Pathways

• Ovulation
• Oocyte Maturation
• Pathogenesis
• Cell Proliferation
• Luteinization
• Metaphase
• Gene Silencing
• Immune Response
• Cell Cycle
• Methylation
• Cell Growth
• Rna Interference
• Cell Differentiation

• Regeneration
• Fertilization
• Reverse Transcription
• Estrus
• Demethylation
• Angiogenesis
• Cell Adhesion

PTMs

• Phosphorylation
• Methylation

• Demethylation
• Reduction

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EREG

Boster Bio Antibodies - Best Uses Of The EREG Marker

There are a variety of methods to use antibodies against the EREG marker. Boster Bio offers primary antibodies as well as troubleshooting and optimization guides and ELISA kits. Listed below are the best applications for Boster Bio antibodies for the EREG marker. Boster Bio is available to answer any questions you may ask or to provide more information. We're eager to hear from you!

Boster Bio's primary antibody

Boster Bio's main antibody is an extremely specific marker that can identify proteins. This is among the advantages of using them. The EREG marker is a common marker that is found in human tissues and is highly regulated in the brain. The EREG marker assists researchers in identifying proteins that code for genes, which are vital for brain function. Boster Bio's primary antibodies utilize EREG and GAPDH to identify EREG signaling pathways.

Optimization guides

The Boster Bio immunohistochemistry Optimization Guide includes protocols, troubleshooting tips and suggestions for preparation of samples. The guide addresses common problems with immunohistochemistry, including weak staining, high background, and nonspecific staining. This guide is intended for researchers and provides an extensive overview of all available resources. Boster has chosen a handful of important protocols and techniques for researchers to follow. These guides are an excellent resource for scientists seeking the highest sensitivity and maximum results.

ELISA kits

ELISA kits for EREG proteins are a reliable method to determine the concentrations in biological fluids. These kits are extremely sensitive and specific and are excellent for cancer research. The kits are currently employed to determine the concentration of Human EREG in plasma, serum and other biological fluids. The range of concentrations for EREG is 0.2-100ng/mL.

ELISA methods employ high-affinity antibodies to detect specific proteins in crude preparations. These high-affinity antibodies can also be used to determine small amounts of analytes inside complex samples. ELISA kits for EREG markers are more sensitive than colorimetric tests because of their specificity. Chemiluminescent detection could have different signal intensities.

The MBS2703932 is an easy-to-use, microwell-strip-plate ELISA kit designed to detect Epiregulin (EREG) protein in biological samples. The ELISA kit uses the proprietary SimpleStep ELISA(r) technology to analyze EREG in biological samples. The ELISA microplates of the kit are designed to detect EREG in undiluted body fluids, tissue homogenates, and secretions.

The ELISA test can be used to determine whether the EREG protein is expressed in a particular cell type. Indirect detection of the protein requires the biotin-streptavidin compound or a secondary antibody. In sandwich ELISAs, both the primary and secondary antibodies are pre-coated. The secondary antibody is then cross-bound to the capture antibody. Based on the type of signal the plate can be clear or transparent.

EREG is a key protein found in tumor cells, triggering NF-CAF transformation in a JAK2-STAT3-dependent manner. EREG is also crucial in the role of promoting invasion of fibroblasts during cancer, and its overexpression in tumor cells is believed contribute to the progression of OSCC. Therefore, research based on EREG requires the use of ELISA kits for the EREG marker.

EREG expression was higher in CAFs and NFs. CAFs are vital to the invasion of HSC3 cells, and the overexpression of EREG significantly increased the capacity of these cells to assist in HSC3 invasion. AG490 is a drug that inhibits JAK2-STAT3, was able restore CAF invasion. Contrarily, CAFs' overexpression of EREG increased their ability to aid in invasion.