Click image to see more details
Product Info Summary
| SKU: | EK0543 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Human VEGFR1/sFLT1 ELISA Kit PicoKine®
View all VEGFR1/Flt-1 ELISA kits
SKU/Catalog Number
EK0543
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human VEGFR1/sFLT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human VEGFR1/sFLT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0543)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S27-H687
Sensitivity
<30 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0543 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0543 is reactive to FLT1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0543 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR1/Flt-1
sVEGFR1, also known as sFMS-related tyrosine kinase 1 (sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0543-CAP | Anti-Human FLT1 Pre-coated 96-well strip microplate | 1 |
| EK0543-ST | Human FLT1 Standard | 2 vials, 10 ng/tube |
| EK0543-DA | Human FLT1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.031 | 0.069 | 0.124 | 0.164 | 0.366 | 0.681 | 1.178 | 1.920 |
Data Example Images
Click image to see more details
Human VEGFR1/sFLT1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR1/Flt-1, For serum, dilution ratio is 1:1 and concentration is 3.6-5 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is less than the lowest standard, 156 pg/ml..
Some articles we found to cite concentrations of VEGFR1/Flt-1 in samples: 16449946 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VEGFR1/sFLT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 237 | 1369 | 4420 | 240 | 1352 | 4316 |
| Standard deviation | 9.71 | 106.78 | 203.32 | 11.28 | 131.14 | 202.85 |
| CV (%) | 4.1% | 7.8% | 4.6% | 4.7% | 9.7% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFR1/Flt-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 237 | 212 | 228 | 228 | 226 | 9.01 | 3.9% |
| Sample 2 | 1369 | 1318 | 1326 | 1352 | 1341 | 20.36 | 1.5% |
| Sample 3 | 4420 | 4343 | 4598 | 4779 | 4535 | 168.51 | 3.7% |
Protein Target Info & Infographic
Gene/Protein Information For FLT1 (Source: Uniprot.Org, NCBI)
Gene Name
FLT1
Full Name
Vascular endothelial growth factor receptor 1
Weight
150769 MW
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 1;VEGFR-1;2.7.10.1;Fms-like tyrosine kinase 1;FLT-1;Tyrosine-protein kinase FRT;Tyrosine-protein kinase receptor FLT;FLT;Vascular permeability factor receptor;FLT1;FLT, FRT, VEGFR1; FLT1 FLT, FLT-1, VEGFR-1, VEGFR1 fms related receptor tyrosine kinase 1 vascular endothelial growth factor receptor 1|fms related tyrosine kinase 1|fms-like tyrosine kinase 1|fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)|tyrosine-protein kinase FRT|tyrosine-protein kinase receptor FLT|vascular permeability factor receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FLT1, check out the FLT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human VEGFR1/sFLT1 ELISA Kit PicoKine® (EK0543)
Hello CJ!
EK0543 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum Vascular Endothelial Growth Factor and Vascular Endothelial Growth Factor Receptor-1 Levels in Patients With Fibromyalgia Syndrome
Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene
Attenuation of hyperglycemia-induced apoptotic signaling and anti-angiogenic milieu in cultured cytotrophoblast cells
Soluble Fms‐like Tyrosine Kinase‐1 Expression Inhibits the Growth of Multiple Myeloma in Nude Mice
Soluble vascular endothelial growth factor receptor‐1 as a novel marker of arteriovenous fistula stenosis in hemodialysis patients
Vascular endothelial growth factor gene polymorphisms in patients with rosacea: A case-control study
Protective effect of human umbilical cord mesenchymal stem cell exosomes on preserving the morphology and angiogenesis of placenta in rats with preeclampsia
Upregulation of sFlt-1 by trophoblasts induces the barrier dysfunction of glomerular endothelial cells
Effects of maternal serum on permeability of glomerular endothelial cell membrane
Liu M,Wang RB,Xing JH,Tang YX.Nested Case–Control Study of Corin Combined with sFlt-1/PLGF in Predicting the Risk of Preeclampsia.Int J Gen Med.2021;14:2313-2320https://doi.org/10.2147/IJGM.S297344
Species: Human
EK0543 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human VEGFR1/sFLT1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human VEGFR1/sFLT1 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Human VEGFR1/sFLT1 ELISA Kit PicoKine®
Question
I have a question regarding this product. Could you please provide me with the immunogen sequence for capture and detector antibody? If it is not possible to provide the sequence itself, could you please send me the amino acid range that the immunogen is from? Or if possible, provide the percentage alignment of the immunogen sequence with mouse? I would appreciate it if you could just tell me if this kit can be used with a mouse or not.
Verified customer
Asked: 2022-10-10
Answer
EK0543 doesn't cross react with mouse and rat samples. The immunongen sequence of the antibody pair in EK0543 is S27-H687(Accession # NP_001153392).
Boster Scientific Support
Answered: 2022-10-10
Question
Is EK0543 specific for sFLT-1 or can it detect FLT-1 as well?
Verified customer
Asked: 2022-04-11
Answer
The Human SVEGFR1/SFLT1 ELISA Kit PicoKine (EK0543) is a solid-phase immunoassay specially designed to measure Human FLT1 with a 96-well strip plate that is pre-coated with antibody specific for FLT1. The detection antibody is a biotinylated antibody specific for FLT1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human FLT1 with immunogen: Expression system for standard: NS0; Immunogen sequence: S27-H687. The kit is analytically validated with ready-to-use reagents.
Boster Scientific Support
Answered: 2022-04-11
Question
What serum dilution do you suggest for measuring sFlt1 in serum samples of severe preclamptic and eclamptic patients using EK0543?
Verified customer
Asked: 2020-11-12
Answer
For the Human sVEGFR1/sFLT1 ELISA Kit (EK0543), our lab tested serum from normal healthy humans with dilution of 1:1. We suggest starting with 1:1. Please, run pilot tests with his specific samples to figure out the optimal dilution ratio.
Boster Scientific Support
Answered: 2020-11-13
Question
Does EK0543 react with rat and mouse samples?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
EK0543 will not react with rat and mouse samples.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: Can VEGFR1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Liu
Verified customer
Asked: 2019-08-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-08-03
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
B. Wright
Verified customer
Asked: 2019-04-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-04-09
Question
Will EK0543 also react with Rat sFLT1?
Verified customer
Asked: 2019-03-12
Answer
The Human SVEGFR1/SFLT1 ELISA Kit PicoKine™ (EK0543) will not react with rat sFLT1.
Boster Scientific Support
Answered: 2019-03-12
Question
Q: can you recommend the dilution ratio of serum samples for detection of VEGFR1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFR1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Miller
Verified customer
Asked: 2018-09-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-09
Question
Do you know if EK0543 will react with rat and mouse samples?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
EK0543 will not react with rat and mouse samples.
Boster Scientific Support
Answered: 2018-04-19
Question
a customer who purchased EK0543 requests if you have any suggestion or how many hours after the blood collection they should proceed with serum separation in order to analyze the samples using your kit?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
It takes four hours
Boster Scientific Support
Answered: 2017-10-12
Question
Why is there such a large concentration range of 156-10000 pg/ml for the Human sVEGFR1/sFLT1 PicoKine™ ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The range has been set to take into account that this detects a growth factor
Boster Scientific Support
Answered: 2017-10-12
Question
Does EK0543 detected bound or unbound VEGF-A?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
EK0543 is able to detect both bound and unbound SVEGFR1/SFLT1.
Boster Scientific Support
Answered: 2017-07-27
Question
Q: is it okay to use heparin plasma as samples in Human VEGFR1 Picokine® ELISA Kit (Catalog # EK0543)?
Verified Customer
Verified customer
Asked: 2016-09-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VEGFR1 causing degradation of its protein structure. VEGFR1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the VEGFR1 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for VEGFR1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-09-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFR1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-09-02
