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Product Info Summary
| SKU: | A01756-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD2AP DyLight® 488 conjugated Antibody
SKU/Catalog Number
A01756-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD2AP DyLight® 488 conjugated Antibody catalog # A01756-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD2AP DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01756-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01756-Dyl488 is reactive to CD2AP in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
70.45kDa
Background of Cd2ap
CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01756-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of A431 cells using anti-Human CD2AP antibody (A01756-Dyl488).
Overlay histogram showing A431 cells stained with A01756-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human CD2AP Antibody (A01756-Dyl488, 1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CD2AP (Source: Uniprot.org, NCBI)
Gene Name
CD2AP
Full Name
CD2-associated protein
Weight
70.45kDa
Alternative Names
CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP Cd2ap|AL024079, C78928, METS-, METS-1, Mets, Mets1|CD2-associated protein|CD2-associated protein|SH3 domain-containing adapter protein|SH3 domain-containing adaptor protein METS-1|mesenchyme to epithelium transition protein, SH3 domains|mesenchyme-to-epithelium transition protein with SH3 domains 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD2AP, check out the CD2AP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD2AP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD2AP DyLight® 488 conjugated Antibody (A01756-Dyl488)
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