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Product Info Summary
| SKU: | PA1399 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VEGF Receptor 1/FLT1 Antibody Picoband®
View all VEGFR1/Flt-1 Antibodies
SKU/Catalog Number
PA1399
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF Receptor 1/FLT1 Antibody catalog # PA1399. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF Receptor 1/FLT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1399)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FLT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1399 is reactive to FLT1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
151 kDa
Calculated molecular weight
150769 MW
Background of VEGFR1/Flt-1
Vascular endothelial growth factor receptor 1 (FLT1) is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family. It is mapped to 13q12. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. It has a 758-amino acid extracellular domain, followed by a 22-amino acid transmembrane region and a 558-amino acid cytoplasmic region containing a cluster of basic amino acids and a tyrosine kinase domain. sFLT-1 was identified in placenta, adult lung, kidney, liver and uterus. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1399 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Cell, SGC Cell, MM231 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-FLT1 antibody, PA1399, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: SGC Cell Lysate
Lane 3: MM231 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For FLT1 (Source: Uniprot.org, NCBI)
Gene Name
FLT1
Full Name
Vascular endothelial growth factor receptor 1
Weight
150769 MW
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 1;VEGFR-1;2.7.10.1;Fms-like tyrosine kinase 1;FLT-1;Tyrosine-protein kinase FRT;Tyrosine-protein kinase receptor FLT;FLT;Vascular permeability factor receptor;FLT1;FLT, FRT, VEGFR1; FLT1 FLT, FLT-1, VEGFR-1, VEGFR1 fms related receptor tyrosine kinase 1 vascular endothelial growth factor receptor 1|fms related tyrosine kinase 1|fms-like tyrosine kinase 1|fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)|tyrosine-protein kinase FRT|tyrosine-protein kinase receptor FLT|vascular permeability factor receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLT1, check out the FLT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF Receptor 1/FLT1 Antibody Picoband® (PA1399)
Hello CJ!
PA1399 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Wu Y, You H, Ma P, Li L, Yuan Y, Li J, Ye X, Liu X, Yao H, Chen R, Lai K, Yang X. Plos One. 2013 May 9;8(5):E62827. Doi: 10.1371/Journal.Pone.0062827. Print 2013. Role Of Transient Receptor Potential Ion Channels And Evoked Levels Of Neuropeptides...
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-VEGF Receptor 1/FLT1 Antibody Picoband®
Question
Will anti-VEGF Receptor 1/FLT1 antibody PA1399 work on bovine WB with umbilical vein?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Our lab technicians have not tested anti-VEGF Receptor 1/FLT1 antibody PA1399 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-VEGF Receptor 1/FLT1 antibody PA1399 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine umbilical vein in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
Would PA1399 anti-VEGF Receptor 1/FLT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
As indicated on the product datasheet, PA1399 anti-VEGF Receptor 1/FLT1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-17
Question
I have a question about product PA1399, anti-VEGF Receptor 1/FLT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1399 anti-VEGF Receptor 1/FLT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-28
Question
We are interested in to test anti-VEGF Receptor 1/FLT1 antibody PA1399 on human umbilical vein for research purposes, then I may be interested in using anti-VEGF Receptor 1/FLT1 antibody PA1399 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-VEGF Receptor 1/FLT1 antibody PA1399, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
Would anti-VEGF Receptor 1/FLT1 antibody PA1399 work for WB with umbilical vein?
J. Zhao
Verified customer
Asked: 2015-03-18
Answer
According to the expression profile of umbilical vein, FLT1 is highly expressed in umbilical vein. So, it is likely that anti-VEGF Receptor 1/FLT1 antibody PA1399 will work for WB with umbilical vein.
Boster Scientific Support
Answered: 2015-03-18
Question
I see that the anti-VEGF Receptor 1/FLT1 antibody PA1399 works with WB, what is the protocol used to produce the result images on the product page?
H. Edwards
Verified customer
Asked: 2014-02-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-02-28
