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Product Info Summary
| SKU: | A01756-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD2AP DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01756-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD2AP DyLight® 550 conjugated Antibody catalog # A01756-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD2AP DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01756-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01756-Dyl550 is reactive to CD2AP in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
70.45kDa
Background of Cd2ap
CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01756-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD2AP (Source: Uniprot.org, NCBI)
Gene Name
CD2AP
Full Name
CD2-associated protein
Weight
70.45kDa
Alternative Names
CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP Cd2ap|AL024079, C78928, METS-, METS-1, Mets, Mets1|CD2-associated protein|CD2-associated protein|SH3 domain-containing adapter protein|SH3 domain-containing adaptor protein METS-1|mesenchyme to epithelium transition protein, SH3 domains|mesenchyme-to-epithelium transition protein with SH3 domains 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD2AP, check out the CD2AP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD2AP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD2AP DyLight® 550 conjugated Antibody (A01756-Dyl550)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Human CD2AP DyLight® 550 conjugated Antibody
Question
Do you have a BSA free version of anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-10
Question
Does anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 work on bovine Flow Cytometry with platelet?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not validated anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine platelet in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Human CD2AP DyLight® 550 conjugated antibody for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
It shows on the product datasheet, A01756-Dyl550 anti-Human CD2AP DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-10
Question
I see that the anti-Human CD2AP DyLight® 550 conjugated antibody A01756-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
R. Mitchell
Verified customer
Asked: 2016-11-04
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-11-04
Question
Would A01756-Dyl550 anti-Human CD2AP DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Anderson
Verified customer
Asked: 2013-03-29
Answer
As indicated on the product datasheet, A01756-Dyl550 anti-Human CD2AP DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-29
