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Product Info Summary
| SKU: | EK1631 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Serpin A5/PAI3 ELISA Kit PicoKine®
View all Serpin A5/Protein C Inhibitor ELISA kits
SKU/Catalog Number
EK1631
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin A5/PAI3 ELISA Kit PicoKine® (96 Tests). Quantitate Human Serpina5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin A5/PAI3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1631)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E26-P406
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1631 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1631 is reactive to Serpina5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1631 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin A5/Protein C Inhibitor
Protein C inhibitor (PCI), also known as SERPINA5, is serine protease inhibitor of serpin type that is found in most tissues and fluids, including blood plasma, seminal plasma and urine of human. It is a 52kD glycoprotein and belongs to serine protease inhibitor (Serpin) super family of protein. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. This gene is mapped on the q arm of chromosome 14.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1631-CAP | Anti-Human Serpina5 Pre-coated 96-well strip microplate | 1 |
| EK1631-ST | Human Serpina5 Standard | 2 vials, 10 ng/tube |
| EK1631-DA | Human Serpina5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.048 | 0.135 | 0.279 | 0.415 | 0.669 | 1.138 | 1.654 | 1.936 |
Data Example Images
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Human Serpin A5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin A5/Protein C Inhibitor, Dilution ratio of 1:500, concentration in serum and plasma is around 600 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Serpin A5/PAI3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 225 | 1716 | 44577 | 220 | 1814 | 45770 |
| Standard deviation | 17.77 | 127.96 | 314.23 | 20.9 | 137.86 | 407.35 |
| CV (%) | 7.9% | 7.3% | 7% | 9.5% | 7.6% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin A5/Protein C Inhibitor in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 225 | 227 | 232 | 228 | 228 | 2.54 | 1.1% |
| Sample 2 | 1716 | 1679 | 1599 | 1655 | 1662 | 42.49 | 2.5% |
| Sample 3 | 4489 | 3979 | 4488 | 4123 | 4269 | 224.59 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For Serpina5 (Source: Uniprot.Org, NCBI)
Gene Name
Serpina5
Full Name
Plasma serine protease inhibitor
Weight
45.675kDa
Superfamily
serpin family
Alternative Names
Plasma serine protease inhibitor; Acrosomal serine protease inhibitor; Plasminogen activator inhibitor 3; PAI-3; PAI3; Protein C inhibitor; PCI; Serpin A5; SERPINA5; PCI; PLANH3; PROCI SERPINA5 PAI-3, PAI3, PCI, PCI-B, PLANH3, PROCI serpin family A member 5 plasma serine protease inhibitor|acrosomal serine protease inhibitor|plasminogen activator inhibitor III|plasminogen activator inhibitor-3|protein C inhibitor|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Serpina5, check out the Serpina5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpina5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin A5/PAI3 ELISA Kit PicoKine® (EK1631)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human Serpin A5/PAI3 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin A5 using the EK1631 Human Serpin A5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-09-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-09-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-07-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-12
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Serpin A5 level in plasma .
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-18
Question
What are the Clone IDs of both the capture and detection polyclonal antibodies?
Verified customer
Asked: 2019-04-30
Answer
We do not have Clone IDs for polyclonal antibodies. Clone IDs are for monoclonal antibodies.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-08-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-08-04
Question
Q: how to thaw whole blood sample for Serpin A5 ELISA after freezing?
T. Bhasin
Verified customer
Asked: 2018-05-07
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin A5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-06
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Serpin A5 in Human plasma ? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-03-24
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin A5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-24
Question
Q: if the enzyme conjugated Serpin A5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin A5 antigen?
L. Dhar
Verified customer
Asked: 2014-11-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-11-01
