Key=store_id
Key=entity_id, value=47831
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EZ0386
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2018-10-15 12:31:20
Key=updated_at, value=2024-09-26 00:58:37
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Key=cj_search_index
Key=publication_count, value=3
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=ez-set
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1365
Key=yoast_content_score, value=60
Key=price, value=500.0000
Key=weight, value=1.0000
Key=meta_title, value=Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Key=meta_description, value=Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IGFBP3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml.
Key=image, value=/e/k/ek0386.jpg
Key=small_image, value=/e/k/ek0386.jpg
Key=thumbnail, value=/e/k/ek0386.jpg
Key=url_key, value=human-igfbp3-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0386-boster
Key=url_path, value=human-igfbp3-elisa-kit-ez-set-trade-diy-antibody-pairs
Key=options_container, value=Product Info Column
Key=image_label, value=Human IGFBP3 EZ-Set ELISA Kit standard curve
Key=small_image_label, value=Human IGFBP3 EZ-Set ELISA Kit standard curve
Key=thumbnail_label, value=Human IGFBP3 EZ-Set ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=For 5 plates, 96 wells each
Key=gene_full_name, value=Insulin-like growth factor-binding protein 3;IBP-3;IGF-binding protein 3;IGFBP-3;IGFBP3;IBP3;
Key=uniprot_id, value=P17936
Key=assay_range, value=156 pg/ml - 10,000 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=anticoagulant, value=heparin or EDTA
Key=tmb_incubation_time, value=25-30min
Key=source_company, value=Boster EZ0386
Key=product_category, value=EZ-Set ELISA Kits (Ab Pairs)
Key=research_category, value=Cell Biology, Cell Death, Diabetes-associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases
Key=swatch_image, value=/antibody/ek0387-2-ELISA-mouse-igfbp-3-picokine-elisa-kit.jpg
Key=rating_value, value=98.3
Key=review_count, value=10
Key=mp_exclude_sitemap, value=Yes
Key=capture_antibody, value=Rabbit anti- human IGFBP3 polyclonal antibody
Key=detection_antibody, value=Biotinylated goat anti- human IGFBP3 polyclonal antibody
Key=cj_related_products, value=Growth Hormone Secretion:EK0387|A00435-2|PROTP17936-2|PROTP17936|PA1694|A04188
Key=description, value=Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IGFBP3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=short_description, value=Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IGFBP3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=background, value=Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.
Key=gene_name, value=IGFBP3
Key=synonyms, value=Acid stable subunit of the 140 K IGF complex; Binding protein 29; Binding protein 53; BP 53; BP53; Growth hormone dependent binding protein; IBP 3; IBP3; IGF binding protein 3; IGFBP 3; Insulin Like Growth Factor Binding Protein 3;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0386-CA	Rabbit anti- human IGFBP3 polyclonal antibody (Capture Antibody)	500 µl, 0.3 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0386-DA	Biotinylated goat anti- human IGFBP3 polyclonal antibody (Detection Antibody)	500 µl, 2 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0386-ST	Lyophilized recombinant human IGFBP3 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	271	1607	5480	253	1639	5548
Standard deviation	16.53	69.1	350.72	19.73	95.06	438.29
CV (%)	6.1%	4.3%	6.4%	7.8%	5.8%	7.9%

Key=componentsneeded, value=

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered.
10. Color Developing Reagent: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T).
  PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered.
  PBS-T: 0.1% Tween? 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Key=subcellular_localization, value=Secreted
Key=tissue_specificity, value=Expressed by most tissues. Present in plasma.
Key=protein_function, value=IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Key=sample_data, value=

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.034	0.092	0.16	0.23	0.397	0.691	1.251	1.71

Key=description_before_attributes, value=

Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: A28Q-291
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=preparation_step_2, value=1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10 ng of lyophilized human IGFBP3 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10 ng/ml using 1 ml of Reagent Diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Human IGFBP3 Standard
• Number tubes 1 - 8. Final Concentrations to be Tube # 1 - 10,000 pg/ml, # 2 - 5,000 pg/ml, # 3 - 2,500 pg/ml, # 4 - 1,250 pg/ml, # 5 - 625 pg/ml, # 6 - 312 pg/ml, # 7 - 156 pg/ml, # 8 - 0.0 (Blank).
• For standard # 1, add 1,000 µl of undiluted standard stock solution to tube # 1.
• Add 300 µl of Reagent Diluent to tubes # 2 - 7.
• To generate standard # 2, add 300 µl of standard # 1 from tube # 1 to tube # 2 for a final volume of 600 µl. Mix thoroughly.
• To generate standard # 3, add 300 µl of standard # 2 from tube # 2 to tube # 3 for a final volume of 600 µl. Mix thoroughly.
• Continue the serial dilution for tube # 4 - 7.
• Tube # 8 is a blank standard to be used with every experiment.
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=quantity_and_stock_status
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images